Conventional PCR assays for the detection of pathogenic Vibrio spp. in shrimp aquaculture in the Philippine
Journal Title: Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society - Year 2011, Vol 4, Issue 3
Abstract
Shrimp culture is considered as one of the major industries in most aquaculture-producing countries in Asia. This industry has grown steadily over the years, but its gradual decline is due to several disease outbreaks of either bacterial or viral in origin. Luminous vibriosis, which is caused mainly by Vibrio harveyi has been implicated for mass mortalities in shrimp aquaculture in the Philippines. In addition, another pathogen, V. cambellii also causes vibriosis in shrimp, but this bacterium does not exhibit luminescence. The use of molecular methods particularly the polymerase chain reaction (PCR), facilitated early detection of the causative agent; thus, control measures have been undertaken to ensure efficient management strategies, thus preventing massive mortalities of the cultured stock. This paper reviews the different PCR protocols that have been developed for early detection of pathogenic Vibrio spp. in Philippine shrimp aquaculture. These PCR protocols were developed by targeting specific genes of the pathogen in order to ensure accurate diagnosis of the disease. In addition, the use of direct colony PCR, multiplex PCR and co-amplification together with another viral pathogen is also discussed.
Authors and Affiliations
Christopher Caipang, Mary Aguana
Keywords
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