Correlation between Aspergillus fumigatus isolates recovered from human and broiler chickens
Journal Title: Journal of Veterinary Medical Research - Year 2019, Vol 26, Issue
Abstract
A total of 225 samples were collected from suspected cases and they were 75 human samples (40 ear swabs, 29 vaginal swabs and 6 sputum samples) and 150 from broiler chickens for microbiological examination and fungal isolation, they collected from different areas in El-Fayoum and BeniSuef governorates, from which 129 fungal isolates were recovered, 22 fungal isolates( 29.3%) were recovered from human; of which 15 isolates of ear swabs ( A.fumigatus 37.5%) while 7 (24.1%) A.fumigatus isolates were recovered from women, but there was no A.fumigatus recovered from sputum samples, as well as there were 53 A.fumigatus isolates (35.3%) recovered from broiler chicken. The antifungal activities of thymol and carvacrol oils against the recovered fungal isolates were tested using agar dilution method. Thymol and carvacrol oils completely inhibited the growth of different fungal isolates at concentrations of 1% and 0.1%. On the other hand, the concentration of 0.01% was too weak to inhibit the fungal growth, but it completely reduced the colour of the fungal colony converted it into white coloured arial mycelium. PCR assay using oligonucleotide primer that amplifying 250bp fragment in its Gene of A.fumigatus and A.niger was performed. Sequence analysis of two isolates of A.niger and A.fumigatus using its Gene was performed.
Authors and Affiliations
Radwan I. A. , Kamel M. F. , Hamdy D. A. , Mahmoud Z. A.
Keywords
Related Articles
- EP ID EP657540
- DOI -
- Views 378
- Downloads 0
Potential risk of some pathogens in table eggs
This study was conducted to record the potential risk of some pathogens in table eggs in Beni-Suef city, Egypt. A total of 100 table eggs samples (Farm and Baladi eggs) were randomly collected from poultry farms, markets...
Some pharmacodynamic interactions between salinomycin and vitamin E or selenium in chickens
The present study was conducted to determine the effect of salinomycin at a concentration 60 and 120 ppm alone and with vitamin E or selenium on haematological and biochemical parameters and histopathological changes of...
EFFECT OF VIRGIN OLIVE OIL SUPPLEMENTATION ON SOME HEMATOLOGIC AND THYROID HORMONES, LEVELS IN RATS
The aim of the present study is to investigate the effect of virgin olive oil on some blood parameters in male Albino rats. Thirty male Sprague Dawley rats, (90-110 g), were used in the present study and were divided int...
Direct detection and identification of mycobacteria in bovine samples by PCR assay
Bovine tuberculosis, caused by Mycobacterium bovis, is a zoonotic disease causing approximately 6% of total human deaths. Its economic losses are not only a reduction of 10-20% in milk production and weight, but also inf...
Ultrasonography of the mammary gland in ruminants
Information regarding the use of ultrasonography in the diagnosis of udder and teat affections is scarce in ruminant. The present study was conducted on 30 cows, 20 ewes and 45 does. The surgical affections of udder and...