Correlation between type Ⅱ CD36 deficiency and intron polymorphism
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 8
Abstract
Objective To study the correlation between type ⅡCD36 deficiency and the polymorphism of intron sequence. Methods A total of 516 random healthy platelet donors from Liaoning Blood Center were involved: 241 of them were tested for CD36 antigen and CD36 gene sequence; the remaining 275 cases were sequenced only. CD36 antigen was detected by flow cytometry, and gene sequence was analyzed by PCRamplification and sequencing. Results Among the 241 samples, 1 case of type Ⅰ deficiency and 4 cases of type Ⅱ deficiency were detected, with frequencies of 0.41% and 1.66%, respectively. There was no nucleotide change in the coding region of 3 cases of typeⅡdeficiency. All individuals with type Ⅱ deficiency shared a common polymorphism in the intron 3, that is, carried (TG) 11 and 12 linked variants, and both were located in the same allele. The gene frequency of (TG)11 in the 516 random population was only 11.72%, which was much lower than 30.43% of (TG)13. The gene frequency of 12 linked variants in the random population was 8.81%. Almost all 12 linked variants occurred simultaneously with (TG)11, but only about 72.7% of (TG)11 were tandem with 12 linked variants. Flow cytometry showed that the expression of CD36 antigen on platelet in samples carrying only (TG)11 was comparable to that of normal samples, while the vast majority of samples carrying both (TG)11 and 12 linked variants had significantly lower CD36 antigen levels. Conclusion The (TG)11 in the intron 3 region is not platelet-specific silent allele, but there are some indirect correlations. There may be multiple platelet-specific silent allele.
Authors and Affiliations
Fengqiu LIN, Xiaofeng LI, Jianping LI
Keywords
Related Articles
- EP ID EP738310
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.08.003
- Views 27
- Downloads 0
Preoperative therapeutic plasma exchange to prevent acute rejection after ABO incompatible renal transplantation: a retrospective analysis
Objective To investigate the clinical efficacy of preoperative therapeutic plasma exchange(TPE) in preventing acute rejection after ABO incompatible kidney transplantation(ABOi-KT). Methods Nine patients with ABOi-KT who...
HBV laboratory tests and demographic characteristics in 169 897 first-time blood donors
Objective To investigate the HBV infection markers detection and demographic characteristics of first-time blood donors, so as to provide evidence for blood donor recruitment. Methods HBsAg was detected by ELISA, HBsAg n...
Correlation between serological screening of human T-lymphotropic virus antibodies and confirmatory tests
Objective To explore the correlation between serological screening of human T-lymphotropic virus antibodies (anti HTLV) and Western blot(WB) confirmatory tests among blood donors, so as to explore the infection status of...
Clinical blood dispatching between hospitals in Shijiazhuang
Objective To analyze the blood dispatching of hospitals in Shijiazhuang area, in order to provide reference for blood dispatching policy of hospitals and ensure clinical blood supply. Methods The hospital blood dispatchi...
Genotyping identification and sequence analysis of ABO ambiguous blood group in 20 cases
Objective To study the genotypes of ABO ambiguous blood group samples(n=20) and identify their molecular biological characteristics. Methods The serological phenotype of the samples was analyzed by serological techniques...