Costus Afer Stem Juice Extract Enhances Wound Healing Process Through the Modulation of the Expression of TGF-Β1 at Wound Site
Journal Title: Journal of Regenerative Medicine & Biology Research - Year 2024, Vol 5, Issue 3
Abstract
Recent studies have explored the potential of Costus afer stem juice in supporting the process of tissue repair and regeneration. This study sought to expand on those discoveries by assessing the expression of transforming growth factor beta 1 (TGF-β1) in wounds treated with C. afer stem juice extracts. Ninety-six adult Wistar rats, divided into four groups, were used to study the wound healing process over 24, 72 and 120 hours, as well as at complete healing. The rats were wounded on their dorsum and treated on the first day according to their group: Group A received no treatment, Group B was treated with honey gel, Group C with heat-dried C. afer stem juice extract and Group D with freeze-dried C. afer stem juice extract. The result of the study showed that the expression of TGF-β1 was initially highest in group D which gradually declined to the lowest at 120 hours and peaked again in the healed tissues. Group C wounds showed the lowest level of expression of TGF-β1 at 24 hours, which increased at 72 hours and gradually fell to be at the same level as group B and group A wounds in the healed tissues. The deposition of granulation tissues and extracellular matrix fibres in group D followed the same pattern as expression of TGF-β1. In conclusion, the extract of C. afer stem juice enhanced the healing process through the modulation of TGF-β1 expression.
Authors and Affiliations
Kudighe P Udoh1*, Akpan U Ekanem2, Utomobong U Akpan3, Eno-Obong I Bassey2
Keywords
Related Articles
- EP ID EP748610
- DOI https://doi.org/10.46889/JRMBR.2024.5301
- Views 27
- Downloads 0
Informed Consent Guidelines for Optimizing the Use of Telomerase-Positive Stem Cells
The objectives of the work, based on previous characterization studies, pre-clinical animal models of induced diseases, e.g., Parkinson disease, cardiovascular disease, pulmonary disease, and type-I diabetes mellitus,...
Human CO Model of Creutzfeldt - Jakob Disease (CJD) Evaluates the Effect of Putative Drugs on Prion Accumulation
Creutzfeldt Jakob Disease (CJD) is incurable and fatal neurodegenerative disease. The treatment in human is cell based models for prion disease. An induced pluripotent stem cell based models of human cerebral organ has s...
Neutrophils and Neovascularization
Neovascularization (NV) via growth of collateral arteries (arteriogenesis) and capillaries, arterioles and venules (angiogenesis) becomes impaired as ischemia from vascular disease progresses [1-3]. Our hypothesis is tha...
Preparation of Nanofat Concentrate and Its Use for Hypertrophic Scar Treatment
Objective: Nanofat grafting has been reported to improve tissue repair by the Stromal Vascular Fraction (SVF) of nanofat. However, the oil and liquid portions of fat tissues were released by mechanical emulsification dur...
Telomerase-Positive Stem Cells in Adult Porcine and Adult Rat Spleens I. Totipotent Stem Cells
Previous studies have reported the presence of endogenous undifferentiated totipotent stem cells within the organs and tissues of various animal species, including the spleen. Since one major function of the spleen is...