CRI SPR/Cas: applications and perspectives for plant genetic improvement
Journal Title: Biotecnología Vegetal - Year 2018, Vol 18, Issue 3
Abstract
The rapid development of CRISPR/Cas technology in recent years, from its discovery as a microbial immune system to its evolution as a versatile and powerful tool for the modification of gene function, constitutes a statement on how basic research with a moderate impact can revolutionize biological investigations. From its application as a platform for RNA-guided gene editing, the CRISPR/Cas system has spread to fields as diverse as the regulation of gene expression, epigenome editing, and in vivo visualization of chromatin. In this article the CRISPR/ Cas technology is introduced, with emphasis on the CRISPR/Cas9 type II system and its main applications in plant breeding are discussed. Likewise, advances and limitations in the use of this technology in plants are highlighted, and future perspectives in the field of genetic improvement are outlined.
Authors and Affiliations
Mairenys Concepción-Hernández
Keywords
Related Articles
- EP ID EP682975
- DOI -
- Views 156
- Downloads 1
Effect of heat treatment at high temperature on in vitro seeds germination of Phaseolus vulgaris cv. ‘ICA Pijao’
The common bean (Phaseolus vulgaris L.) production is limited by biotic and abiotic factors. Among them, water stress, saline or high temperatures. To solve this problem, genetic improvement programs are aimed at the s...
Effect of Sodium Chloride on the in vitro development of cultivars 'Gran enano' and 'FHIA 03'
The effect of Sodium Chloride levels on in vitro development of Gran Enano (AAA) and `FHIA 03`(AABB) cultivars was determined. Researches were carried out at Instituto de Investigaciones en Viandas Tropicales (INIVIT)...
Gerbera jamessonii H. Bolus micropropagation
Gerbera is one of the horticultural crops of great demand on the Cuban local market, but its exploitation is limited because with the traditional multiplication methods established, it is not possible to comply with the...
Plantain (Musa spp. cv. ‘Navolean’ AAB) transgenic plants from Agrobacterium tumefaciens-mediated transformation of embryogenic cell suspensions
The present work was carried out with the objective to obtain plantain transgenic plants of ‘Navolean’ (Musa AAB) cultivar and to evaluate them, under field conditions during the first crop cycle, for Black Sigatoka di...
Effect of CO 2 on Coffea arabica L. cv. ‘Caturra rojo’ and Clematis tangutica K. somatic embryos development
Studies to optimize somatic embryogenesis have traditionally focused on the components of the culture medium but little other in vitro environment factors have been analyzed such as the composition of the gaseous atmos...