CRI SPR/Cas: applications and perspectives for plant genetic improvement
Journal Title: Biotecnología Vegetal - Year 2018, Vol 18, Issue 3
Abstract
The rapid development of CRISPR/Cas technology in recent years, from its discovery as a microbial immune system to its evolution as a versatile and powerful tool for the modification of gene function, constitutes a statement on how basic research with a moderate impact can revolutionize biological investigations. From its application as a platform for RNA-guided gene editing, the CRISPR/Cas system has spread to fields as diverse as the regulation of gene expression, epigenome editing, and in vivo visualization of chromatin. In this article the CRISPR/ Cas technology is introduced, with emphasis on the CRISPR/Cas9 type II system and its main applications in plant breeding are discussed. Likewise, advances and limitations in the use of this technology in plants are highlighted, and future perspectives in the field of genetic improvement are outlined.
Authors and Affiliations
Mairenys Concepción-Hernández
Keywords
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