CROWN-ETHER INFLUENCE ON RAT BLOOD PLASMA CYTOKINES AND IMMUNOGLOBULINES CONCENTRATIONS AND PROTEIN PEROXIDATION
Journal Title: Біологія та валеологія - Year 2018, Vol 0, Issue 20
Abstract
The present paper illustrates the experimental results of investigation of immunoglobulins, cytokines, Schiff’s bases and 2.4-dinitrophenylhydrazones blood plasma and serum concentrations in rats organism subjected to intoxication by crown-ethers. The research program used sub-acute toxicological experiment on sexually mature white male rats of WAG population (body mass – 180-220 g). The animals were administered with water emulsion of investigated crown-ethers (12-crown-4, 15-crown-5, 18-crown-6, and aza-12-crown-4) in 1/100 daily (0.0117, 0.0135, 0.0127, 0.022 g/kg respectively), within 30 days per orally. The animals of the control group were given water at the same conditions. On the 30th day of the experiment the rats of all groups were anesthetized by sodium thiopental (50 mg/kg) and slaughtered by decapitation with the Guillotine knife. Cytokine (interleukins-4, -8 (ILs-4, -8), tumor necrosis factor-alpha (TNF-α)) and immunoglobulins (IGA, IGG, IGE, IGM) blood plasma concentrations were determined by immunoenzyme method with application of correspondent standard reagents «Protein Contour» (Saint-Petersburg, Russia) and an immunoenzyme analyzer Stat Fax 303 Plus. Blood serum Schiff’s bases were extracted by Falch mixture (chloroform-methanol) with the following spectrofluorometric analysis in chloroform extract using initiating wave length of 360 nm and emission wave length of 430 nm. Evaluation of blood serum protein oxidative modification intensity was performed by Dubinina’s modified spectrofluorometric method, which is based on an interaction reaction between oxidized protein amino acids residues and 2.4-dinitrophenylhydrazine with the formation of 2.4-dinitrophenylhydrazones (DNPHs). The latter were registered using wave length of 356 (for neutral aldehyde-DNPHs), 370 (for neutral ketone-DNPHs) and 430 (for basic aldehyde-DNPHs) nm. The action of crown-ethers resulted in the significant reduction (25% on average) of IGA concentration in rat blood plasma compared to the control magnitudes, diminishing the very first link of organism immune defense, and potentially being a cause of virus and bacteria growth enhance in the organism. Crown-ethers effects were also displayed in significant decrease in IGG blood plasma concentration (in 45, 40, 38, 30 % for 12-crown-2, 15-crown-5, 18-crown-6 and aza-12-crown-4, respectively), non-specifically inhibiting the producing function of B-cells and which may signify the exhaustion of this class antibodies defensive role. These results very well correspond to the decrease in IGE rat blood plasma concentrations at the influence of the investigated chemical agents. The less decrease in this index in percentage compared to the percentage decrease in IGG concentration (30, 25, 28, 20% for 12-crown-2, 15-crown-5, 18-crown-6 and aza-12-crown-4, respectively) may signify mostly the disturbance of B-cells immune link in the action of the investigated substances. Crown-ethers action upon the rat organism resulted in significant decrease in IL-4 blood plasma concentration (in 38, 33, 30, 25% for 12-crown-2, 15-crown-5, 18-crown-6, and aza-12-crown-4, respectively). Simultaneously, crown-ethers influence upon IL-8 blood plasma concentration was displayed in the significant raise of the index in 34, 30, 27, and 22% for 12-crown-2, 15-crown-5, 18-crown-6 and aza-12-crown-4, respectively. The experiments for blood serum Schiff’s bases contents determination revealed the significant increase in this index in the organism of all experimental groups animals. Obviously, Schiff’s bases are intermediate products of amino acids decarboxilation and transamination reactions. Therefore, the increase in their blood serum concentration may suggest the excessive proteolysis in tissues and involvement of amino acids in different metabolic pathways. The action of crown-ethers 1.5-2 fold increased the blood serum neutral aldehydedinitrophenylhydrazones, and 1.3-1.5 fold increased neutral ketone-DNHPs. These alterations were accompanied by the 2-2.3 raise in basic aldehyde-DNHPs blood serum concentration in the organism of experimental group animals. Significant changes in the levels of basic aldehyde-DNHPs may be connected with the enhanced process of protein glycozylation at conditions of oxidative stress.
Authors and Affiliations
R. I. Kratenko
Keywords
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- EP ID EP513139
- DOI 10.5281/zenodo.2543501
- Views 128
- Downloads 0
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