Curcumol Targets Gls1 to Regulate Glutamine Metabolism and Induce Senescence of Hepatic Stellate Cells to Exert Anti-Hepatic Fibrosis Mechanism
Journal Title: Immunological Disorders and Immunotherapy - Year 2023, Vol 8, Issue 1
Abstract
Objective: Curcuma has long been used as a Chinese herbal medicine for the treatment of chronic liver disease, and curcumol is its main active ingredient. This study explores the molecular mechanism of curcumol regulating glutamine metabolism, hoping to provide new ideas for the prevention and treatment of chronic liver disease. Methods: We used LPS to activate hepatic stellate cells. The effects of curcumol on glutamine metabolism and cell proliferation were detected by kit. The effects of curcumol on GLS1, senescence-related proteins and extracellular matrix expression were observed by Western blotting and RT-PCR. The effect of curcumol on cell cycle was detected by flow cytometry. Furthermore, after overexpression of GLS1, we observed the effects of curcumol on glutamine metabolism, expression of related molecules and cell cycle. Results: Curcumol significantly inhibited cell proliferation, glutamine metabolism, GLS1 and extracellular matrix expression, and significantly promoted cell senescence. When we further over express GLS1, the inhibitory effect of curcumol on glutamine metabolism and the effect of promoting cell senescence were also saved. Conclusions: Curcumol targets GLS1, inhibits glutamine metabolism and induces senescence of hepatic stellate cells, which may be one of the important mechanisms of its anti-hepatic fibrosis. GLS1 may be an important target site for the prevention and treatment of chronic liver disease.
Authors and Affiliations
Xuelin Duan, Tiejian Zhao, Jiaru Wang, Jiahui Wang, Yang Zheng
Keywords
Related Articles
- EP ID EP743044
- DOI 10.35248/2593-8509.23.8.135
- Views 43
- Downloads 0
Bilateral Choroidal Metastasis as Initial Presentation of Parotid Gland Adenoid Cystic Carcinoma
Choroidal metastasis rarely reveals a primary cancer. At this stage, they are associated with multiple metastases. Adenoid cystic carcinoma also known as cylindroma is very rare and constitutes 1% of major and minor sali...
A Study to Investigate the Role of GULP/ CED 6 Gene in “Eat Me” Signaling in Cellular Efferocytosis and Immunosurveillance
In this report, investigations were done to study human GULP/ CED 6 genes role in presenting cancer cells to scavenger cells. CED 6 SiRNA was used to knock out the gene in Astrocytoma (HTB-12) cell lines to study its eff...
P-Selectin is a Key Molecule Underlying the Pathophysiology of AspirinExacerbated Respiratory Disease
Recently, the evidence for platelet association with allergic diseases has been established by many researches. Activated platelets contribute to airway hyper reactivity, bronchoconstriction, airway inflammation and airw...
Unique COVID/Anti-Spike Protein Immune Responses in Two Elderly Patients without COVID-19 Vaccination
Published reports continue to emerge regarding different health outcomes in patients immunized and not immunized against the SARS-CoV-2 antigen (i.e., spike protein) that were infected with the virus. Because of the vast...
https://www.longdom.org/open-access/differential-gene-expression-of-threatened-himalayan-snow-trout-challengedwith-aeromonashydrophilla-84987.html
Objective: Curcuma has long been used as a Chinese herbal medicine for the treatment of chronic liver disease, and curcumol is its main active ingredient. This study explores the molecular mechanism of curcumol regulatin...