CYTOTOXIC ACTIVITY OF A MEDICINAL MANGROVE PLANT CERIOPS DECANDRA (GRIFF.) DING HOU LEAVES ON HUMAN BREAST ADENOCARCINOMA CELL LINE MCF-7
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2018, Vol 11, Issue 12
Abstract
Objective: Ceriops decandra (Griff.) Ding Hou (Rhizophoraceae) is an evergreen mangrove tree widely used in folklore medicine for the treatment of several diseases. Concerning the presence of anticancer molecule in C. decandra, the present investigation was carried out to study the preliminary phytochemical screening, in vitro cytotoxic effect, and apoptotic induction of methanol extract of C. decandra.Methods: The preliminary phytochemical investigations were done using standard methods. Cytotoxic activity was analyzed in terms of brine shrimp (Artemia salina Leach) lethality bioassay. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay was used to evaluate the cell viability and growth inhibition of MCF-7 (human breast adenocarcinoma) cells after treatment with different doses of C. decandra extract. Cell morphological changes induced by the extract were observed using phase contrast inverted microscope. Induction of apoptosis was determined by DNA fragmentation assay.Results: Phytochemical screening revealed the presence of various potent phytochemicals. In the brine shrimp lethality bioassay, the LC50 of the extract was found to be 12.5 μg/mL, implying a promising cytotoxic effect. The MTT assay results showed increased inhibitory activity at a concentration-dependent manner and altered the cell morphology after exposure with C. decandra extract which exhibited typical apoptotic morphological changes. Furthermore, the characteristic DNA ladder pattern of C. decandra treated cells in agarose gel electrophoresis confirms the induction of apoptosis.Conclusion: The research revealed the cytotoxic effect of C. decandra on MCF-7 cells through its nature of induction of apoptosis.
Authors and Affiliations
Sukasini S, Bhargav Iyer M
Keywords
Related Articles
- EP ID EP605212
- DOI 10.22159/ajpcr.2018.v11i12.25635
- Views 100
- Downloads 0
PREVALENCE OF FUNGAL IN OPPORTUNISTIC INFECTION HIV TYPE-1 IN SUBURBAN, KADAPA
Objective: Isolate fungal element causing in opportunistic infection of human immunodeficiency virus and correlated with CD4 cell counts.Methods: Different samples are used and media: Sabouraud dextrose agar, using mycol...
ETHANOLIC EXTRACT OF TUBTIM-CHUMPHAE RICE BRAN DECREASES INSULIN RESISTANCE AND INTRAHEPATIC FAT ACCUMULATION IN HIGH-FAT-HIGH-FRUCTOSE DIET FED RATS
Objective: Tubtim-chumphae rice is hybrid Thai rice with a red pericarp. This study was aimed to investigate the effect of Tubtim-chumphae rice bran on insulin resistance and intrahepatic fat accumulation in high-fat-hig...
CYTOTOXIC EFFECT OF PEPTIDE FUNCTIONALIZED SILVER NANOPARTICLES SYNTHESIZED FROM ALOIN ON BREAST CANCER CELL LINE
Objective: The aim of the present study is to synthesize the silver (Ag) nanoparticle using Aloin with a focus on its antibacterial, antioxidant, and anticancer activity. Methods: The silver nanoparticles were synthesiz...
PHARMACOLOGICAL ACTIVITY OF ULVA LACTUCA POLYPHENOLS FRACTION: HEPATOPROTECTIVE AND ANTIOXIDANT ACTIVITIES AGAINST PARACETAMOL-INDUCED LIVER DAMAGE IN RATS
Objective: The objective of this study was to assess the activity Ulva lactuca polyphenols fraction in protecting the liver damage induced by high dose of paracetamol. Methods: This study was performed using Wistar albi...
EVALUATION OF ANTIBACTERIAL, ANTIOXIDANT, AND ANTICANCER POTENTIALS FROM MARINE RED ALGAE GRACILARIA CORTICATA
Objective: The present study was to evaluate the biological activity of Red algae, Gracilaria corticata, collected from the southeast coast of Mandapam, Ramanathapuram, Tamil Nadu. Seaweeds are rich in bioactive compound...