PLEIOTROPIC ROLE OF SIMVASTATIN AND ALENDRONATE ON MESENCHYMAL STEM CELLS
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2018, Vol 11, Issue 12
Abstract
Objectives: Previously, our research group has investigated the effect of alendronate (ALN) and simvastatin (SV) in their minimum inhibitory dose (IC50) on oral squamous cell carcinoma cell line where inhibition of angiogenesis has been demonstrated. In the present study, we further investigate the effect of the previously calculated IC50 of SV and ALN and their combination on two different types of stem cells to show that the same drug may have different effects on different cells.Methods: Stem cells were isolated from rat adipose tissue and oral mucosa. After passaging, cells were subjected to Aln, Sv separately as well as combined in their half maximal inhibitory concentration (IC50). 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay was performed to evaluate cytotoxicity. After seven days, osteogenic differentiation was evaluated using alizarin stain. Real time polymerase chain reaction was used to evaluate Osteopontin gene expression.Results: Our results demonstrated that the proposed combination of Aln and Sv in their IC50 enhanced the osteogenic differentiation of both types of stem cells.Conclusion: The combined effect of Aln and Sv may represent a novel pharmacological approach in treating bone metastasis and at the same time treating the cancer itself.
Authors and Affiliations
Riham M Aly, Marwa M Ellithy, Dina Sabry
Keywords
Related Articles
- EP ID EP605271
- DOI 10.22159/ajpcr.2018.v11i12.29697
- Views 99
- Downloads 0
PHYTOCHEMICAL CONSTITUENTS OF THE ROOT OF BERBERIS MICROPHYLLA
Objective: To objective of this work was to perform phytochemical qualitative and quantitative analyzes of the main secondary metabolites in the root of Berberis microphylla.Methods: The extracts of B. microphylla root w...
PICA WITH AN UNUSUAL FORM OF PRESENTATION: A CASE REPORT
ABSTRACTGeophagia is a condition involving the consumption of mud and is a form of eating disorder known as pica. Pica is an unusual craving for ingestion ofeither edible or inedible substances. Although, pica is not lin...
EFFECTS OF CINNAMOMUM ZEYLANICUM BARK EXTRACT ON NOCICEPTION AND ANXIETY LIKE BEHAVIOR IN MICE
Objectives: The aim of the study was to assess the effect of the extract of Cinnamomum zeylanicum (CZ) bark in the experimental models of pain and anxiety-like behavior in mice. Methods: The extract of CZ bark was admin...
ANTIOXIDANT, ANTIBACTERIAL, AND ANTICANCER ACTIVITY FROM MARINE RED ALGAE GRACILARIA EDULIS.
Objective: The marine seaweed Gracilaria edulis was collected from Rameshwaram Mandapam coast. The main objective of G. edulis was to check about the antioxidant, antibacterial, and anticancer activity. The potential use...
PYRAZOLINES AS POTENT ANTITUBERCULAR AND CYTOTOXIC AGENTS
Objective: Pyrazolines are known to exhibit different biological and pharmacological properties such as anticancer, antibacterial, antifungal and antitubercular activities. Chalcones with an enone group between two aroma...