Cytotoxic and Genotoxic Biomonitering In Patients Exposed To Radiation Therapy - A Cytological Study
Journal Title: Indian Journal of Dental Advancements - Year 2015, Vol 7, Issue 4
Abstract
Context: Carcinogenesis is a multistep process characterized by genetic, epigenetic and phenotypic changes. Such changes involve genetic damage, mutation in critical genes related to control of cell division, cell death and metastatic potential. The use of radiation therapy to treat cancer inevitably involves exposure of normal tissues. As a result, patients may experience symptoms associated with damage to normal tissue during the course of therapy for a few weeks after therapy or months or years later. Aims & Objectives: The present study is aimed to investigate DNA damage in oral mucosal cells of patients exposed to radiotherapy by the micronucleus test. Cytotoxic effects, pyknosis, condensed chromatin, karyorrhexis and karyolysis are also evaluated in the present study. Materials & Methods: Smears were collected before radiation and during the radiation therapy from first week to sixth week. The smears were fixed with absolute alcohol, stained with PAP and were examined under light microscope. Results: The results showed micronuclei on lesional side of buccal mucosa with karyorhexis and occasional pyknosis on both lesional and opposite side before radiation. Conclusion: Increased cytotoxic & genotoxic effects in oral mucosal cells seen in patients exposed before and after 1st two weeks of radiotherapy. The effects gradually decreased after 4th week radiotherapy, suggesting positive response to radiotherapy.
Authors and Affiliations
Sushma Susik M, Shyam Prasad Reddy D, Keerthi M
Keywords
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- EP ID EP507789
- DOI 10.5866/2015.7.10246
- Views 84
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