Cytotoxic and Genotoxic Effects of Aqueous Extracts of Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. on in vitro Human Peripheral Blood Lymphocytes
Journal Title: Cumhuriyet Science journal - Year 2018, Vol 39, Issue 1
Abstract
The aim of this study was to investigation of in vitro cytotoxic and genotoxic effects of infusion and decoction aqueous extracts obtained from Rosmarinus officinalis, Lavandula stoechas L. and Tilia cordata Mill. on human peripheral blood lymphocytes. For this purpose, lymphocytes taken from 3 females, were treated with aqueous extracts of R. officinalis (1.5 and 3 mg/ml), L. stoechas (0,4 and 1,2 mg/ml) and T. cordata (1 mg/ml and 3 mg/ml) for 48 hours. PBS was used as negative control and Mitomycin C (MMC) (0,25 μg/ml) was used as positive control in experiments. Cytotoxic effect of extracts on lymphocytes was determined by calculating mitotic index. Also, chromosome aberrations micronucleus formations were determined. All extracts considerably decreased the cell division in lymphocytes depending on the increased concentration in comparison with control groups (p<0,01). Also cells in metaphase decreased. Thus, no chromosomal aberration was observed in lymphocytes. However, extract treatments induced the formation of micronucleus in lymphocytes when compared to control groups (p<0,01). 3 mg/ml decoction extracts of L. stoechas and T. cordata showed the highest cytotoxic effect on lymphocytes, and the highest genotoxic effect appeared after 48 h treatment with 1.2 mg/ml decoction extract of L. stoechas.
Authors and Affiliations
Tülay AŞKIN ÇELİK, Özlem Sultan ASLANTÜRK
Keywords
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- EP ID EP488479
- DOI 10.17776/csj.405629
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