CYTOTOXICITY AND PHYTOCHEMICAL STUDIES OF PUMPKIN SEED (CUCURBITA MAXIMA LINN.) EXTRACT.
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2014, Vol 3, Issue 2
Abstract
Pumpkin (Curcurbita maxima) is a popular vegetable in our country. This plant is locally known as “Mistikumra”.Pumpkin (Cucurbita sp.) seeds are a key food source for humans because they are a very good source of proteins (24−36.5%) and oil (31.5−51%)Pumpkin seeds have long been valued as an important natural food for men’s health. Pumpkin seed extract is useful for immunomodulation, reproductive health and therapeutic advantage over a wide range of disease conditions. The intake of a whole extract of pumpkin seeds has been correlated with reduced benign prostate hyperplasia-associated symptoms. The antioxidative property of pumpkin seed extract could also improve fertility, and it helps to prevent arteriosclerosis, high blood pressure and heart diseases; it also stimulates metabolism of accumulated fats.It has potential for allergic reactions to pumpkin that is side effect of pumpkin seeds. Cytotoxic effect of Pumpkin (Curcurbita maxima) seed was studied against brine shrimp nauplii. In brine shrimp lethality bioassay, the LD50 value of methanol extracts and petroleum ether fraction of Pumpkin (Curcurbita maxima) seed were determined and found to be 31.70 ppm and 21.95 ppm respectively. As the petroleum ether fraction showed greater cytotoxic effect we made further study with this fraction. The petroleum ether fraction was subjected to thin layer chromatography on slica gel that yielded four fractions designated as PE-1, PE-2, PE-3, and PE-4, among them only PE-4 was suitable for further purification because it gave single band on two dimensional TLC. The PE-4 may be steroid because it showed pink colour after spraying vanillin sulfuric acid. The IR data revealed that the isolated PE-4 compound contained aromatic ring and ketonic groups (-CO-). NMR and Mass spectral analysis is further needed to elucidate the molecular structure of the compound. The experiments showed that it has a cytotoxic effect and IR data proved that it is steroid compound.
Authors and Affiliations
Shahangir Biswas| Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh, Ahsan Habib| Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh, M. Manirujjaman| Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh-Department of Biochemistry, Gono Bishwabidyalay( Gono University) Savar, Dhaka, Bangladesh, Belal Uddin| Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh, M. M. H. Khan| Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh, Sohel Hasan| Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh, Meftah Uddin| Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh, Minarul Islam| Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh, M. Khatun| Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh, M. A. Islam| Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh, Matiar Rahman*| Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh
Keywords
Related Articles
- EP ID EP2419
- DOI -
- Views 414
- Downloads 20
RELATION OF ANTIOXIDANT STATUS AND LIPOPROTEIN (a) LEVEL IN MYOCARDIAL INFARCTION PATIENTS : A CLINICAL STUDY TO EVALUATE THE ANTIOXIDANT/ LIPOPROTEIN (a) RATIO AS A NOVEL PARAMETER TO INDICATE CARDIOVASCULAR ACCIDENT
Lipoprotein (a), a prominent marker of cardiovascular accident, was first described by Berg in 1963(3). Lipoprotein (a) [Lp(a)] has been considered as one of the distinguished signature in myocardial infarction and for m...
TO STUDY SUSPECTED ADVERSE REACTIONS INCLUDING BIRTH DEFECTS IN PRIVATE APEX HEALTH CARE CENTRE (UTTAR PRADESH) INDIA.
According to the World Health Organization definition, this is any noxious, unintended, and undesired effect of a drug, which occurs at doses used in humans for prophylaxis, diagnosis, or therapy. This definition exclude...
A STUDY OF ANTI-HYPERLIPIDEMIC ACTIVITY OF MARKETED FORMULATIONS OF TERMINALIA ARJUNA POWDER USING EXPERIMENTAL ANIMAL MODEL
Hyperlipidemia is a disorder of lipid metabolism which is major risk of coronary heart disease. Now a day’s synthetic drugs have been associated with number of side effects but herbal medicines have lipid lowering and an...
Prescribing Pattern of Antibiotics in Intensive Care Unit of a Tertiary Care Teaching Hospital
Antibiotics are the most commonly administered drugs in ICU. By optimizing the use of antibiotics within ICU, patient outcomes are improved, better initial antibiotics administration is provided, and the chance of furthe...
A REVIEW ON ROLE OF PERIDURAL FIBROSIS AND ITS DIAGNOSIS
FBSS is a challenging clinical entity with significant impact on the individual and society. To better prevent and manage this condition, knowledge of the factors contributing to its development is necessary. While resea...