CYTOTOXICITY EVALUATION OF TITANIUM AND ZINC OXIDE NANOPARTICLES ON HUMAN CELL LINES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 10
Abstract
Objective: In vitro cytotoxicity evaluation of titanium dioxide, 20 nm (TNP 20) and zinc oxide, 20 nm (ZNP 20) nanoparticles (NP) were tested on different types of human skin (HaCat), lung (A549), liver (Hep G2) and colon (Caco-2) cell cultures in relevance to human risk assessmentMethods: The different concentrations of test TNP 20 and ZNP 20 1-300 µg/ml were exposed to determine the cell viability reduction on four human cell lines after 48 h post exposure using 3-(4, 5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). The mitochondrial membrane activities of the viable cells were determined with intensity of formazon formation by interpreting ELISA absorbance values at 470 nm.Results: The percent of cytotoxicity was determined by comparing percentage of cell viability reduction of test with that of control. The ZNP 20 produced higher cytotoxicity at the doses 100 (p<0.05) and 300 (p<0.001) µg/ml significantly on tested four human skin (HaCaT), lung (A549), liver (Hep G2) and colon (Caco-2) cells compared to TNP 20. The tested NP induced lesser cytotoxicity at lower concentrations with 1 and 3µg/ml in all the tested four cell lines. The induced cytotoxicity was an indicator for increased intracellular reactive oxygen species which further cause’s major cell damage and cell death.Conclusion: The tested NP were induced greater cytotoxicity in the colon, Liver, lung and skin cells at higher concentrations 100 and 300 µg/ml significantly. The cytotoxicity order of TNP 20 and ZNP 20 at the highest dose (300µg/ml) were concluded as Caco-2>Hep G2>A549>HaCaT for 48 h post exposed cells.
Authors and Affiliations
Durgaiah Gandamalla, Harikiran Lingabathula, Narsimha Reddy Yellu
Keywords
Related Articles
- EP ID EP575478
- DOI 10.22159/ijpps.2017v9i11.21924
- Views 54
- Downloads 0
EVALUATION OF THE PROTECTIVE EFFECT OF ETHANOLIC EXTRACT OF LEAVES OF PUNICA GRANATUM LINN. ON FORCED SWIMMING INDUCED CHRONIC FATIGUE SYNDROME IN MICE
Objective: To study the protective effect of ethanolic extract of leaves of Punica granatum Linn. (EEPG) on forced swimming induced chronic fatigue syndrome (CFS) in mice.Methods: Male albino mice of 25-40 grams were gro...
EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF STEM EXTRACTS OF CUSCUTA REFLEXA (ROXB) IN RATS
Objective: Cuscuta Reflexa (Convolvulaceae) is a plant with a variety of ethnic medicinal uses along with antioxidant activity. Hence it was planned to evaluate the hepatoprotective activity with alcoholic extracts of st...
DETERMINATION OF HYDROLYSIS PARAMETERS OF YOHIMBINE HCl AT NEUTRAL AND SLIGHTLY ACIDIC MEDIUM
Objectives: In the process of investigating various new drug delivery systems of yohimbine HCl (Yoh), it was necessary to study some of the physical chemical properties of the drug including its stability at neutral, aci...
A RAPID RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION OF EPLERENONE IN TABLETS
Objective: To develop a rapid, sensitive, accurate, precise, linear and rugged Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method and validate as per ICH guidelines for the quantitative estimation of...
BIOPROCESS MODELING FOR THE PREDICTION OF THERAPEUTIC ENZYME L-ASPARAGINASE ACTIVITY IN SOLID STATE FERMENTATION USING MULTIPLE LINEAR REGRESSION AND ANN
Objective: L-asparaginase is an enzyme of industrial as well as therapeutic importance. The capabilities of bioprocess modeling of L-Asparaginase activity produced from Aspergillus niger by solid state fermentation (SSF)...