D, L Glyceraldehyde- Will it be an effective Antiglycolytic Agent?
Journal Title: International Journal of Clinical Biochemistry and Research - Year 2016, Vol 3, Issue 4
Abstract
Aim: This study aimed to compare the effectiveness of D, L Glyceraldehyde (GLY) with Plain Sodium Fluoride (NaF) as an antiglycolytic agent and its interferences in the estimation of Serum creatinine by Modified Jaffe′s method and Serum Sodium and Potassium by ion selective electrode(ISE) method. Materials and Methods: Eighty random blood samples were collected from the OP patient after obtaining explicit consent. Serum Glucose, Creatinine, Sodium and Potassium were estimated in all the three tubes and the results were compared across. Statistical Analysis: ANOVA test was done to compare the analytical values obtained from all the three tubes. Results: Mean Glucose concentration in Plain Tube with Clot activator (PTC), NaF tube and the GLY tube were 142.25 mg/dl, 124.41mg/dl and 139.23mg/dl respectively. p-value for GLY tube vs NaF was 0.483, GLY tube vs PTC was 0.970 and NaF vs PTC was 0.349(p value < 0.05 considered significant). There was Positive interference with creatinine measurement by modified Jaffe’s method (p<0.0001) and for Serum Potassium estimation by ISE method (p<0.0001). Both sodium and potassium estimation in NaF tube showed significantly increased values when compared to analysis in PTC. Conclusions: For preventing ex vivo glycolysis, D, L-Glyceraldehyde coated tubes is as good as PTC tube for glucose estimation by glucose oxidase peroxidase (GOD-POD) method. D, L Glyceraldehyde coated tubes can be used for the estimation of serum sodium but not for potassium by ISE method and creatinine by Modified Jaffe’s method. PTC tube cannot be replaced by GLY tube for routine chemistry analysis.
Authors and Affiliations
Udaya Kumari Ganapathy, Nagendran Ramachandran
Keywords
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