Database Analysis of Acidic Proteins from Halophilic Species and Their Corresponding Basic Proteins from Non-halophilic Species
Journal Title: Biotechnology Journal International - Year 2016, Vol 14, Issue 2
Abstract
Aims: To reveal which amino acid residues determine whether a protein is acidic or basic between orthologous pairs, acidic proteins from halophilic species and corresponding basic proteins from non-halophilic species were compared. Similarly acidic versus acidic protein pairs, and basic versus basic protein pairs were also analyzed. Place and Duration of Study: Department of Clinical Laboratory Science, Graduate Course of Medical Science and Technology, School of Health Sciences, Kanazawa University, Japan. Methodology: Halobacterium sp. NRC-1 was used as halophilic species and Gram-positive bacterium Bacillus subtilis, and radiation resistant bacterium Deinococcus radiodurans were used as non-halophilic species. The three species were selected because their proteins were closely related each other. The amino acid compositions were compared and the amino acid substitutions were counted for the orthologous protein pairs between Halobacterium and B. subtilis. Similar comparison was done for the proteins between Halobacterium and D. radiodurans. Results: The Asp and Glu residues are determinant whether a protein of Halobacterium sp. NRC-1 is acidic or basic. Amino acid substitutions to increase the Asp residues in the acidic proteins of Halobacterium from the corresponding proteins of non-halophilic species were almost identical whether the corresponding proteins were acidic or basic. This result suggested that the change of protein charges from basic proteins to acidic ones was same as from acidic proteins to acidic ones. The proteins of Halobacterium showed a tendency to have residues with smaller side chain than the proteins of B. subtilis / D. radiodurans.
Authors and Affiliations
Kazuhiro Mawatari, Hiroshi Nakashima, Keiko Homma, Michiko Yamazaki, Masao Ishizaki
Keywords
Related Articles
- EP ID EP237966
- DOI 10.9734/BBJ/2016/25207
- Views 109
- Downloads 0
Direct Multiple Shoot Induction and Plantlet Regeneration from Cotyledonary Explants of Sapindus emarginatus Vahl (Soapnut)
Multiple shoot induction in S. emarginatus has been achieved by two methods: (1) Direct germination of S. emarginatus in vitro cotyledon explants in BAP/Kn/TDZ (1.0-3.0 mg/L) supplemented MS medium and (2) in plant treat...
Optimization and Lipase Production of Lysinibacillus sphaericus in Domestic Oil Rich Waste Water
The present study investigated the growth and lipase production by Lysinibacillus sphaericus in a minimal medium. The growth conditions (i.e oil concentration, nitrogen source, carbon source, metals and non metals) were...
Partial Purification of Cellulase Enzyme from Fusarioum oxysporium by Using Egg White Matrix
Aim: Protein matrix possesses a capacity to bind organic and inorganic matter. Egg white matrix as an adsorbent was utilized for partial purification of cellulase enzyme in detailed batch study. Methodology: Effect of pH...
Response of Pseudomonas species from Contaminated Soils to Selected Organic (Synthetic) Pesticides
Growth response profile of three species of Pseudomonas isolated from pesticide contaminated soils within Uyo, Nigeria was studied using standard microbiological and analytical techniques. The ability of the isolates to...
Gamma Radiation: Physical Elicitor for In vitro Culture Techniques of Cajanus cajan
Gamma rays are very important in mutation breeding and in in vitro mutagenesis in order to develop required features of plants and increase the genetic variability. Cajanus cajan when subjected to absorbed doses 30 Gy, 5...