DECREASEMENT OF LYSOPHOSPHATIDYLCHOLINE LEVEL, NF-KB EXPRESSION, INTIMA MEDIA THICKNESS AND IMPROVEMENT OF INSULIN RESISTANCE BY DARAPLADIB TREATMENT: IN VIVO STUDIES OF TYPE 2 DIABETES MELLITUS SPRAGUE-DAWLEY RAT MODEL
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2017, Vol 10, Issue 12
Abstract
Objective: Lipoprotein-associated phospholipase A2 (Lp-PLA2) is an enzyme with several pro-inflammatory properties that involved in pathogenesis of atherosclerosis, but some investigation shows controversial views regarding its biological role. We examined the effect of selective inhibitor of Lp-PLA2 (darapladib) to the inflammation marker, intima-media thickness (IMT), and insulin resistance (IR) of type 2 diabetes mellitus (T2DM) rat model. This study aimed to measure lysophosphatidylcholine (lyso-PC) in serum and aortic tissue, nuclear factor kappa B (NF-κB) expression, IMT, and IR with darapladib treatment in a T2DM rat model.Methods: 30 Sprague-Dawley rats were randomly divided into normal group, T2DM group and T2DM with darapladib treatment. Induction of T2DM was done by giving high-fat diet and low dose injection of streptozotocin. Blood glucose level and insulin plasma concentration were measured to calculate IR. 8 weeks and 16 weeks after treatment, we compared lyso-PC level, NF-κB expression, and IMT.Results: Darapladib significantly decreased lyso-PC level, NF-κB expression, and IMT at two serial treatments. Darapladib treatment group exhibited significant reduction of IR (0.64±0.11 vs. 2.07±0.16, at 8 weeks; and 0.93±0.08 vs. 6.48±0.55 at 16 weeks) compared with T2DM group.Conclusions: These data suggested that Lp-PLA2 played a role in inflammation process, atherosclerosis, and IR occurring in metabolic disorder.
Authors and Affiliations
Titin Andri Wihastuti, Dinda Zahra Putri Andiyani, Sri Andarini, Teuku Heriansyah
Keywords
Related Articles
- EP ID EP605567
- DOI 10.22159/ajpcr.2017.v10i12.19022
- Views 76
- Downloads 0
DRUG UTILIZATION STUDY IN POST-OPERATIVE PATIENTS IN THE SURGERY WARD OF A TERTIARY CARE TEACHING HOSPITAL IN SOUTH INDIA
Objectives: Pharmacotherapy with multiple agents before, during, and after surgery is marked in the present day indoor hospital setting. The main objective of our research is to evaluate the prescribing and drug utilizat...
EFFECT OF POST-ISOMETRIC RELAXATION AND RECIPROCAL INHIBITION IN OSTEOARTHRITIS KNEE
Objectives : To evaluateeffect of post-isometric relaxation, reciprocal inhibition andcombined effect  post-isometric relaxation and reciprocal inhibition in osteoarthritis knee.Method :Ethical clearance was obtained fr...
STUDY ON MULTIPOTENT MEDICINAL ASPECTS OF SCHIMA WALLICHII (BARK) FROM NAGALAND, NE INDIA
Objective: The present study focuses on the multipotent medicinal aspects of Schima wallichii bark. Methods: The ethanol and water extracts of the plant were investigated its phytochemical, antibacterial, antioxidant, a...
DIABETES MELLITUS TREATMENT: A RAPID REVIEW ON INNOVATIVE THERAPIES
Diabetes mellitus is a chronic and metabolic disorder which results from defects in a section of insulin, action of insulin, or both. Primarily drugs are used to control the symptoms and save life. Secondary aims are to...
AWARENESS, KNOWLEDGE AND ATTITUDE TOWARDS PHARMACOVIGILANCE AMONG MEDICAL GRADUATES IN A TERTIARY CARE TEACHING HOSPITAL IN SOUTH INDIA.
Objectives: As an ever growing scale people are using newer and more effective drugs for various medical conditions. Adverse drug reactions (ADRs) are preventable if the health-care professional pays close attention to t...