Degradation of Cellulosic Substrates By Bacteria Isolated From Earthworm
Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2018, Vol 4, Issue 1
Abstract
A study had been carried out to evaluating degradation ability of cellulosic substrates by bacteria isolated from earthworm. Bacteria were isolated by hungate selective media with Carboxy Methyl Cellulose/CMC as selective substrates. The potency of cellulolytic was identified based on degradation of cellulosic substrates measurement using clear/diffusion zone diameters and cellulase (endoglucanase and exoglucanase) enzyme activities. This study showed that cellulolytic bacteria isolated from earthworm coded EB1CL has produce highest clear/diffusion zone diameters on CMC and rice bran substrates and with highest endoglucanase enzyme activity, while bacteria isolate coded EB8CL has produce highest clear/diffusion zone diameters on avicel and rice straw substrates and with highest exoglucanase enzyme activity. was concluded that bacteria isolates coded EB1CL and EB8CL was potential as cellulosic substrates degrader. bacteria isolates
Authors and Affiliations
Sutama I N. , S. I M. Mudita, I W. Suberata
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