Dengue Diagnostic Test for the Resource Constraint Developing World: Validity of Rapid Immunochromatographic Card Test against ELISA
Journal Title: Saudi Journal of Pathology and Microbiology - Year 2017, Vol 2, Issue 3
Abstract
Abstract: Dengue infection is a serious global public health problem, and the dengue viruses are widely distributed throughout the tropical and subtropical areas of the world. In the Indian subcontinent, the epidemiology of dengue fever has been very complex. High prevalence rate in our region particularly in pre-monsoon and monsoon season is alarming and necessitates early and accurate diagnosis of dengue virus infection to prevent its complications and further morbidity. Now-a-days detection by rapid tests offers an even faster route to a presumptive dengue diagnosis. Hence, this study aims to assess the agreement between the rapid immune-chromatographic card test (RICT) for dengue and antibody capture ELISA, the latter being used as gold standard. A prospective study was conducted from July to November 2014 and July to November 2015 in a secondary care hospital. Probable dengue cases were diagnosed as per the WHO criteria and RICT and ELISA were conducted on the same serum samples for the detection of NS1 antigen and IgM & IgG antibodies. The results were analyzed. The sensitivities of RICT for NS1antigen and IgM & IgG antibodies were 100%, 89.6% and 81.3%, respectively and all showed 100% specificity. In the present study, the accuracy parameters were comparable with ELISA. Also, RICT met the WHO ASSURED criteria, hence this study recommends introduction of RICT in all levels of health care system for an early and accurate diagnosis and confirmation of the clinical suspicion of dengue. Keywords: Dengue, rapid immunochromatographic card test, ELISA, serological markers.
Authors and Affiliations
Pragya Sharma, Yadu Vir Singh, Ajay Kumar, Monisha Biswas, Ashok K Sharma
Keywords
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