Dengue serology–role of NS1 antigen: evaluation and future prospect
Journal Title: International Journal of Medical Research and Review - Year 2016, Vol 4, Issue 8
Abstract
Introduction: According to the estimates, nearly 70% of the disease burden occurs in Asia and is on constant rise. Dengue virus infection has emerged as a major public health concern across the globe in terms of mortality, morbidity and public health cost. Aims & Objectives: This study was undertaken to evaluate the significance of NS1 Ag detection in dengue IgM negative sera as marker for early detection and to compare the rapid detection test kits (RDT’s) with Enzyme linked immunosorbent assay (ELISA). Material & Method: A total of 5283 samples from, were screened for dengue serology by Immunochromatography based lateral flow assay for presence of IgM and IgG anti Dengue antibody and NS 1 Ag. Results of 169 samples were further confirmed using ELISA technique for NS1 Ag in an attempt to compare RDT’s with ELISA. Results: Out of 5283 samples screened, 146/5283 (2.76%) tested positive for dengue antibody, out of which 85/146 (58.21%) were positive for DEN Ig M and 61/146 (41.78%) showed presence of both IgG and IgM. Out of 5137 seronegative samples, 1930 (37.57%) were positive for DEN NS1 Ag. Overall, sensitivity of RDT was 100.00%, specificity-90.16%, PPV- 94.73%, NPV-100.00 % and Efficiency of test-96.44%. Conclusion: NS1 Ag assay holds promise as useful tool in early diagnosis for detecting dengue infection during acute phase of infection when IgM antibodies are not formed to the detectable levels. Also, accurate and affordable diagnostic tests are a crucial component of combating this debilitating mosquito-borne infection.
Authors and Affiliations
Surinder Kumar, Raminder Sandhu, Pallavi Sayal, Shalley Dahiya, 5Diksha Budhani
Keywords
Related Articles
- EP ID EP231139
- DOI 10.17511
- Views 115
- Downloads 0
Effect of progressive muscle relaxation on post-operative analgesia
Background: Postoperative pain begins with surgical trauma, the intensity reduces gradually and ends with tissue recovery. Effective pain management is essential to minimize unnecessary distress and potential complicatio...
Comparison of Universal Plan Indices for Intensity Modulated Radiotherapy of Head and Neck Cancer treatment
Objective: Manual evaluation of IMRT plans for head-and-neck cancers has been especially challenging necessitating efficient and objective assessment tools. Based on previous clinical experience, the radiation oncologist...
Analysis of multiple choice questions (MCQ): important part of assessment of medical students
Background: Assessment influences students learning process that is why analysis of assessment allows us to conduct it properly and accurately. Tarrant M et al. and few others had done similar studies in past. Further st...
Accessory parotid gland: its surgical importance
Introduction: Accessory parotid gland is separated normal tissue from the main parotid gland, lying over the parotid duct. Accessory parotid gland is more prone for parotid tumors than main parotid gland. The accessory p...
A study of clinico-hematological profile of pancytopenic patients in Central India
Background: To segregate the causes of pancytopenia which are easily treatable, from those requiring sophisticated investigations & vigorous treatment, based on clinico-hematological profile of the patients and affordabl...