Design and evaluation of a multi-epitope vaccine for COVID-19: an in silico approach
Journal Title: Health Science Monitor - Year 2023, Vol 2, Issue 3
Abstract
Background & Aims: Corona virus disease-19 (COVID-19) is an evolving global disease which has burst into 2019. SARS-CoV-2 infects human cells through recognition of and binding to angiotensin converting enzyme-2 (ACE2) through the spike (S) glycoprotein. Spike is an immunogenic protein that can elicit immune responses. Multi-epitope vaccines are novel and efficient class of vaccines which are designed by linking the B and T cells. These epitopes stimulate both humoral and cellular immunity. Materials & Methods: Based on bioinformatics online tools, appropriate epitopes of S protein were selected, linked together via suitable linkers, a TLR4 binding adjuvant was added, and a multi-epitope construct was constructed. The 3D model of the construct was predicted, refined, and validated. The antigenicity, allergenicity, solubility, and physico-chemical properties of vaccine were checked. The B cell conformational epitopes and IFN-γ inducing parts were detected. The adjuvant and TLR4 binding were evaluated by docking and protein-protein complex stability was assessed by elastic-mode analysis. The coding sequence of the vaccine construct was optimized and sub-cloned in expression vector through an in silico approach. Finally, the structure, energy, and stability of vaccine coding mRNA were evaluated. Results: Ten continuous B cell epitopes, 9 T helper epitopes, and 8 CTL epitopes were chosen. The results showed that multi-epitope vaccine is a stable and soluble protein which can stimulate humoral and cellular immunity. Besides, the vaccine could stimulate immunity without inducing allergenicity in human body. Conclusion: Finally, the vaccine can bind the TLR4 appropriately and can be expressed by a recombinant vector. The designed multi-epitope vaccine against COVID-19 could be considered as a suitable candidate for experimental studies.
Authors and Affiliations
Mehdi Tourani,Sadra Samavarchi Tehrani,Ahmad Movahedpour,Sahar Rezaei Arablouydareh,Amir Maleksabet,Amir Savardashtaki,Hojat GhasemnejadâBerenji,Mortaza Taheri-Anganeh,
Keywords
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- EP ID EP723590
- DOI http://dx.doi.org/10.61186/hsm.2.3.180
- Views 18
- Downloads 0
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