Design and RP - HPLC Method for the Simultaneous determination of Valsartan and Hydrochlorothiazide in Bulk and in Pharmaceutical Formulation
Journal Title: International Journal of Novel Trends in Pharmaceutical Sciences - Year 2011, Vol 1, Issue 1
Abstract
A reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of valsartan and hydrochlorothiazide in bulk and in pharmaceutical formulation using RP - C18 column. The mobile phase (acetonitrile: methanol: 50 mM phosphate buffer adjusted to pH 3 with orthophosphoric acid) was pumped at a flow rate of 1.0 ml/ min in the ratio of 20: 50: 30%v/v and the eluents were monitored at 250 nm. Linearity was obtained in the concentration range of 4 - 40 g/ ml for valsartan and 1 – 10 g/ ml for hydrochlorothiazide. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method can be applied for determining valsartan and hydrochlorothiazide in bulk and in pharmaceutical dosage form.
Authors and Affiliations
D Jothieswari, S Britoraj, E Mohanambal
Keywords
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