Detectability of tick-borne agents DNA in the blood of dogs, undergoing treatment for borreliosis.
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2009, Vol 16, Issue 1
Abstract
In the wake of controversies surrounding the usefulness of PCR in the diagnostics of borreliosis, the aim of the presented study was to monitor the presence of B. burgdorferi s.l. in dogs with clinical borreliosis in the course of relevant treatment. The monitoring was based on detecting borrelia's DNA before- (study I), during- (study II), and after completion of the therapy (study III). In addition, to rule out possible coinfections, the dogs' blood was examined for the presence of anaplasma, babesia and rickettsia. Blood samples taken from 11 dogs, with clinically detected borreliosis, were used for obtaining DNA for PCR. Positive results of PCR, with primers complementary to the fla gene, indicating the presence of DNA of B. burgdorferi s.l., were noted, in study I, in the blood of 7 dogs (63.6% dogs), in study II in 3 dogs, while in study III all blood samples were negative. In 6 out of 7 PCR+, the first study was carried out during week 1. Therefore, the PCR method is useful for monitoring early canine infections with spirochetes B. burgdorferi s.l. In all positive samples, subjected to PCR-RFLP, it was the case of a single genospecies, i.e. B. burgdorferi sensu stricto. Studies for the presence of DNA of Babesia sp., as well as DNA of Ricettsia helvetica, were negative in all samples. Anaplasma phagocytophilum DNA was detected in the blood of a single dog, and only in study I. The same dog also proved positive for the presence of borrelia DNA. Co-occurrence of both pathogens did not disturb the clinical picture of borreliosis, and the administered treatment was also effective for the mixed infection.
Authors and Affiliations
Beata Wodecka, Anna Rymaszewska, Marek Sawczuk, Bogumiła Skotarczak
Keywords
Related Articles
- EP ID EP58620
- DOI -
- Views 218
- Downloads 0
Factors determining physical activity of Ukrainian students
[b]Objective[/b]. Scientific reports provide information concerning an insufficient level of physical activity of societies. The objective of the study is recognition of the level of physical activity among Ukrainian stu...
Seasonal activity of millipedes (Diplopoda) – their economic and medical significance
The millipede [i]Brachydesmus superus[/i] Latzel, [i]Polydesmus inconstans[/i] Latzel (Diplopoda: Polydesmida) and [i]Kryphioiulus occultus[/i] C. L. Koch (Diplopoda: Julida) were collected from compost in gardens in Lub...
Studies of farmers’ annual exposure to whole body vibration on selected family farms of mixed production profile[sup][/sup]
The objective of the study was to recognize and evaluate the annual exposure of private farmers to whole body mechanical vibration on selected family farms of mixed production profile (plant-animal). The scope of study c...
Exposure to airborne microorganisms and endotoxin in a potato processing plant.
Microbiological air sampling was performed in a big potato processing plant located in eastern Poland. Air samples for determination of concentrations of microorganisms, dust and endotoxin were collected at 6 sites in th...
Can thyroid size still be considered as a useful tool for assessing iodine intake?
Introduction. It has always been very difficult to precisely define a goitre. For years, the borderline values have been sought which could be universally used in such evaluations. However, presented reference values wer...