DETECTION AND MOLECULAR CHARACTERIZATION OF ESBL PRODUCING E. COLI ISOLATED FROM POULTRY DROPPINGS AND ITS ENVIRON.

Journal Title: International Journal of Advanced Research (IJAR) - Year 2019, Vol 7, Issue 8

Abstract

Detection and molecular characterization of ESBL producing E. coli isolated from poultry droppings and its environ was investigated. Samples of poultry droppings (20), air (12), water (12) and soil (12) were collected from four randomly selected poultry farms within Calabar Municipal Local Government Area of Cross-River State. The samples were processed and E. coli isolates were identified using Standard Microbiological Techniques. ESBL phenotypic detection was carried out using Double Disc Synergism Test (DDST) while molecular detection of TEM, SHV and CTX-M genes in the E. coli isolates was carried out using Polymerase chain reaction (PCR). Results obtained revealed that 56 bacteria species were isolated from the samples, out of which 30 (53.57%) were E. coli while 12(21. 43%) were ESBL positive. A higher prevalence of ESBL producing E. coli was observed with samples of the poultry droppings (30%) as compared to that recorded with the air (8.33%), water (16.67%) and soil (25%) samples collected from the poultry farms. TEM, SHV, CTX-M, CTX-M-9 and CTX-M-15 genes were detected in 28.57%, 33.3%, 11.9%, 4.976% and 21.43% of the E. coli isolates while BLa OXA and CTX- M-2 genes were not present in the E. coli strains. However, the study has shown that ESBL producing E. coli circulate within poultry droppings and its environs. Hence, poultry farms are important source for ESBL-producing bacteria which causes difficult-to-treat infections in humans. Therefore, and integrated “one health” surveillance system becomes a necessity in monitoring transmission events and detection of resistant bacteria in a timely manner.

Authors and Affiliations

Andy I. E. , Tiku , D. R. , Okpo E. A. , Mboto C. I. and Utsalo S. J.

Keywords

Related Articles

SCREEN TASK EXPERIMENTS FOR EEG SIGNALS BASED ON SSVEP BRAIN COMPUTER INTERFACE.

Development BCI system based stay state visual evoked potential (SSVEP), require establish the characteristics of the stimuli presented the user for optimal development of the extraction of signal characteristics; for it...

CAUSALITY NEXUS BETWEEN FOREIGN DIRECT INVESTMENT AND ECONOMIC GROWTH: EMPIRICAL EVIDENCE FROM GHANA AND CHINA.

Foreign direct investment (FDI) has always been one of the imperative factors to the economic development of Ghana and China. This paper investigates the impact of foreign direct investment on economic growth using time...

AN ANALYSIS OF STUDENTS? METACOGNITION ABILITY THROUGH JUMPING TASK STRATEGY TO SOLVE GEOMETRY PROBLEM.

This research aimed at analyzing students? metacognition ability through Jumping Task strategy to solve geometry problem. Jumping Task is task-based instruction in which the task is challenging or beyond the standard of...

AGRICULTURE SECTOR: IMPORTANT CHALLENGES AND PROSPEROUS OF INDIAN ECONOMY.

The basic characteristics of underdeveloped countries discussed in research paper might as well be regarded as the obstacles to economic development. Even thought the general characteristics of under development are not...

ROLE OF PHYSICAL THERAPY IN THE MANAGEMENT OF OROFACIAL CANCER.

Aim: To review the existing literature on orofacial cancer and imply the importance of physiotherapy in the management of orofacial Cancer. Methodology: Search strategies from databases like Google Scholar, PubMed, PubMe...

Download PDF file
  • EP ID EP633359
  • DOI 10.21474/IJAR01/9516
  • Views 52
  • Downloads 0

How To Cite

Andy I. E. , Tiku, D. R. , Okpo E. A. , Mboto C. I. and Utsalo S. J. (2019). DETECTION AND MOLECULAR CHARACTERIZATION OF ESBL PRODUCING E. COLI ISOLATED FROM POULTRY DROPPINGS AND ITS ENVIRON.. International Journal of Advanced Research (IJAR), 7(8), 385-392. https://europub.co.uk/articles/-A-633359