Detection of bacterial pathogens in surgical site infections and their antibiotic sensitivity profile
Journal Title: International Journal of Medical Research & Health Sciences - Year 2016, Vol 5, Issue 5
Abstract
Surgical site infections considered as a major problem in health care centers, resulting in extended length of stay, substantial associated morbidity and mortality, and high excess hospital cost. Thirty wound swabs were collected from patients who had developed postoperative wound infections at Rafidia Hospital-Nablus, Palestine. Bacterial isolates were identified according to standard microbiological methods. Antibiotics susceptibility test was applied for all isolated bacterial species. ERIC-PCR was carried out to determine the identity between isolated clones. The results of this research showed that the prevalence of pathogens among surgical site infections was 56.7%, 30%, 6.7%, 3.3% and 3.3% for E. coli, S. aureus, Klebsiella sp., Enterobacter sp., and Acinatobacter sp., respectively. E. coli isolates showed high resistance against Nalidixic acid (88.2%), Trimethoprim/Sulfamethoxazole (76.5%), Tetracycline (70.6%), Norfloxacin (64.7%) and Ciprofloxacin (58.5%). S. aureus showed high resistance against Nalidixic acid (88.9%), Norfloxacin (77.8%), Amoxycillin/clavulanic acid (77.8%), Kanamycin (66.7%) and Ciprofloxacin (55.6%). Methicillin resistant S. aureus (MRSA) accounted for 33.3% of a total of S. aureus isolates. Resistant to 3 or more antibiotics were detected in 94.1% (16/17) and 77.8% (7/9) of E. coli and S. aureus isolates, respectively. ERIC-PCR typing E. coli and S. aureus isolates showed that each was consisted of 4 ERIC-PCR clusters at a 50% similarity level. Indistinguishable and closely related strains were detected for both microorganisms. Results of this study might be important in provoking awareness to postoperative wound infections and further studies are needed to identify other pathogens responsible for SSIs and the source of infections. Using effective antibiotic policy will restrict further spread of postoperative wound infections.
Authors and Affiliations
Ghaleb Adwan
Keywords
Related Articles
- EP ID EP247833
- DOI -
- Views 109
- Downloads 0
Evaluating the effect of genistein on the amount of BDNF protein in the spinal cord of diabetic ovariectomized rats
BDNF (Brain derived neurotrophic factor) is a secretion protein and a member of the neurotrophin family of growth factors. Studies have shown that in ovariectomized animals, the expression of BDNF decreased. However, rec...
Sub-neurolytic dose of phenol for popliteal-sciatic nerve block under ultrasound guidance for the relief of ischemic leg pain
Ischemic leg pain is often intractable. Pain relief with conventional analgesics, opioids and non-opioids is often unsatisfactory. It provides only a sub-optimal pain relief in spite of the grievous side effects it cause...
Study on hepatitis B vaccination coverage among workforce of a tertiary care hospital in North India
Hepatitis B is one of the most serious of the 20 blood borne pathogens which are the major threat to health care workers (HCWs). While a vaccine for hepatitis B exists, its utilization among...
Glycodelin (PAEP) gene promoter polymorphism rs760140467 C>T is associated with recurrent pregnancy loss
In the absence of confirmed causes for around 50% of recurrent pregnancy loss (RPL) cases this study was conducted in order to evaluate the relationship between PAEP gene single nucleotide polymorphism (SNP) rs760140467...
Iatrogenic lumbar Pseudomeningocele: A case report and review of literature
Lumbar pseudomeningocele is an uncommon complication of lumbar spine surgeries. This occurred due to extradural encapsulated cerebrospinal fluid collection after accidental durotomy or un-repairable dural tear. On review...