Detection of bovine herpesvirus type 1 and bovine ephemeral fever virus antigens in cattle lymph nodes using three immunological assays
Journal Title: Journal of Veterinary Medical Research - Year 2005, Vol 15, Issue 2
Abstract
Lymph nodes (Ln) are the preferred samples for virus isolation and detection. In the present study, carcass and visceral Ln of apparently healthy cattle were screened for the presence of bovine herpes virus type 1 (BHV-1) and bovine ephemeral fever virus (BEFV) antigens. A total of 198 Ln (114 carcasses Ln and 84 visceral Ln) were collected. Lymph node homogenates were assayed by agar gel precipitation test (AGPT), rapid Staphyloccocal protein A (SPA) agglutination test and Dot-ELISA. The overall results revealed that BHV-1 antigens were detected in 43.9%, 56.1% and in 68.4% of carcass Ln, and in 29.8%, 47.6% and 57.1% of visceral Ln collected from slaughtered cattle by AGPT, SPA agglutination test and Dot-ELISA respectively. On the other hand, BEFV antigens were detected in 5.3%, 38.6% and 52.6% of carcass Ln, and in 6%, 41.7% and 54.8 % of visceral Ln collected from slaughtered animals by AGPT, rapid SPA agglutination test and Dot-ELISA respectively. The results showed high percentage of positive samples with SPA agglutination test and Dot- ELISA in comparison to AGPT for both BHV-1 and BEF.
Authors and Affiliations
S. M. Tamam; A. S. Abdel-Moneim Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef. Egypt
Keywords
Related Articles
- EP ID EP682720
- DOI -
- Views 202
- Downloads 0
Phenotypic and genotypic characterization of methicillin resistant Staphylococcus aureus
Food contaminated with multiple antibiotic-resistant S.aureus can be a major threat to the public health. The purpose of this study was to isolate S.aureus from different food sources, determine their antimicrobial susce...
Characterization of antimicrobial resistant bacterial pathogens recovered from cases of bovine mastitis with special reference to Staphylococcus aureus
In the current study, a total of 20 and 78 milk samples were collected from animals showed signs of clinical and subclinical mastitis, for isolation and identification of different causative pathogens in some dairy farms...
Some parasites causing diarrhea in goats in Beni-Suef Governorate
A total of 100 fecal samples from goats were coprologically examined to investigate the main cause of diarrhea. Animals were divided according to the age into 3 groups (7-35 days, 35 days - 6month and over one year). The...
The incidence of C. perfringens in chickens in different seasons and Governorates in Egypt
A total of 247 intestinal samples from freshly dead broiler and layer chickens were collected from 150 farms in Giza, Sharkia, Qalubia, El-Behera, Daqahlia and Cairo governorates in different seasons. These samples showe...
Comparison of tramadol, lidocaine and tramadol-lidocaine combination for epidural analgesia in goats
The aim of this study was to compare the analgesic effect of tramadol, lidocaine and tramadol-lidocaine combination injected in the epidural space in goats. Nine goats were used to compare the epidural analgesic effect o...