DETECTION OF DIFFERENT MEAT SPECIES BY MULTIPLEX PCR ASSAY
Journal Title: International Journal of Agriculture Sciences - Year 2016, Vol 8, Issue 52
Abstract
In presence study four meat Species (Buffalo, Sheep, Goat and Chicken) were selected for identification of raw meat. Mitochondrial cytochrome b gene was use as a marker gene for species identification in multiplex PCR. Common forward primers and species-specific reverse primers were used for the development of multiplex PCR by mixing of primers in the different ratio viz. 2.5:1.5:1.5:1.5:3 for forward: Goat: Sheep: Chicken: buffalo, species-specific reverse primers respectively. PCR primers were designed such a way to give different lengths of amplicon for different species, there is 157 bp for goat, 227 bp for chicken, 274 bp for buffalo and 331 bp for sheep in agarose gel electrophoresis, respectively. The present study was undertaken for detection of animal species in meat samples by multiplex PCR assay using cytochrome b gene variability.
Authors and Affiliations
M. D. MAKWANA
Keywords
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