DETECTION OF DNA DAMAGE IN OBESE CARDIOVASCULAR DISEASE PATIENTS AND COMPARISON BETWEEN GENDERS: A CASE-CONTROL STUDY
Journal Title: International Journal of Genetics - Year 2011, Vol 3, Issue 2
Abstract
Background- Obesity and cardiovascular disease (CVD) with the increased stress/ inflammation may cause DNA damage posing risk for malignancy hence assessment of DNA damage gains importance as a pre-cancerous biomarker. Methods-The alkaline Single Cell Gel Electrophoresis assay was carried out in peripheral blood leukocytes of obese-CVD patients (n=28; 14 males and 14 females) and matched controls (n=13; 7 males and 6 females). Multiple regression and analysis of variance were performed for effect of confounding/specific factors on DNA damage and the Student’s t-lest to compare differential values for factors associated with obesity-CVD and DNA damage. Results-In patients, genomic damage was significantly higher (p<0.05): damage frequency (59.21% in males, 60.14% in females), mean DNA migration length (37.53±4.74 μm, 19.16±2.60μm, respectively) in comparison to control data with significantly (p<0.01) increased damage in male patients. The attributes of obese-CVD patients and controls matched except for Body Mass Index (BMI), weight, waist circumference (WC) and waist hip ratio (WHR). Multivariate analysis of variance and multiple regression analysis revealed association of genetic damage with waist and hip circumferences in male patients. The t-test revealed that age, BMI, WHR, WC, cardiovascular disease sub-sets and drug usage had differential significance for genetic damage in female patients. Male patients with arrhythmia had significantly higher DNA damage as those on atenolol-rampril-furosemide therapy. Conclusion- The significantly increased DNA damage in obese-CVD patients may be from the nature of CVD, treatment duration, drug combinations and adiposity and gains importance as it imples a higher risk for age -related diseases and cancer.
Authors and Affiliations
G. GANDHI, J. KAUR, N. MAHAJAN
Keywords
Related Articles
- EP ID EP170159
- DOI 10.9735/0975-2862.3.2.66-71
- Views 103
- Downloads 0
EFFECT OF SALINITY AND DROUGHT STRESS ON GROWTH PARAMETERS, GLYCOSIDE CONTENT AND EXPRESSION LEVEL OF VITAL GENES IN STEVIOL GLYCOSIDES BIOSYNTHESIS PATHWAY OF Stevia rebaudiana (BERTONI)
Stevia [Stevia rebuaidana (Bertoni)], a family of Asteraceae is known to yield diterpenoid steviol glycosides (SGs) that is about 300 times sweeter than sugar. The present work examined the effects of different concentra...
RICE LANDRACES WITH GENETIC VARIATIONS FOR SALINITY TOLERANCE AND THEIR ASSOCIATION WITH SUBMERGENCE TOLERANCE
In coastal regions, both salinity and submergence stress alternatively or simultaneously become severe constrain to the rice cultivation by climate changes. This situation necessitates the search of combined tolerance to...
CHROMOSOMAL Q-HETEROCHROMATIN POLYMORPHISMS IN 3 ETHNIC GROUPS (KAZAKHS, RUSSIANS AND UYGHURS) OF KAZAKHSTAN
A comparative study of polymorphic variants of chromosomal Q-heterochromatin regions (Q-HRs) was performed in three ethnic groups (Kazakhs, Russians, and Uyghurs) of Kazakhstan. The number of chromosomal Q-HRs in the gen...
GENETIC DISSECTION FOR YIELD ATTRIBUTES IN THE FIVE WHEAT CROSSES UNDER TWO SOWING SEASONS
The study was carried out to analyse gene effects for various yield attributes in wheat at CCS Haryana Agricultural University Hissar during rabi 2008-09 and 2009-10. six basic populations (parents, F1, backcrosses and F...
LINKAGE ANALYSIS OF FIRST SIX MODY GENES TO KNOWN LOCI AND SCREENING OF THREE MOST PREVALENT MODY GENES IN PAKISTANI POPULATION
The pandemic of metabolic disorders is accelerating in the urbanized world posing huge burden to health and economy. The key pioneer to most of the metabolic disorders is insulin dependent diabetes mellitus or Type 1 Dia...