Detection of gonorrhea among HIV infected patients by polymerase chain reaction
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2015, Vol 5, Issue 7
Abstract
Objective: To determine the burden of gonorrhea among HIV patients using PCR technique. Methods: A cross sectional study was carried out in a HIV clinic of Sparsha Nepal from January to March, 2011. Standard microbiological procedures were followed during collection and processing of urine samples. DNA amplification, isolation and detection were carried out. Gonorrhoea diagnosis was done by multiplex polymerase chain reaction. Results: Among the 119 HIV positive patients, 12 (10.08%) were positive for N. gonorrheae. The cases were more among the males than in females. The age of patients ranged from 20- 45 years and the highest prevalence was among the age group 20-35 years. The distribution of gonorrhea infected patients according to marital status revealed that 8 cases were among the married patients. Conclusions: This study revealed that the incidence of gonorrhoea was higher in males and in married people. So, the control measures should be targeted to these groups of people.
Retrospective analysis of dengue specific IgM reactive serum samples
Objective: To conduct a retrospective analysis of dengue cases in Kolkata, on the basis of presence of anti-dengue IgM in their sera and presence or absence of anti-dengue IgG and dengue specific Non structural 1 (NS1) a...
Screening for mrsa as a preventive approach prior to nasotracheal intubation
Objective: This study was undertaken to determine the carriage of MRSA strains due to nasotracheal intubation into the lower respiratory tract where they can be a potential source of infection leading to high morbidity a...
Epidemiology and prevention of animal bite and human rabies in a rural community-One health experiment
Objective: To estimate the incidence of human rabies and animal bite/exposure; to describe the post exposure prophylaxis received by animal bite/exposure cases; to assess the safety and immunogenicity of rabies vaccine (...
In vitro screening of snake venom against multidrug-resistant tuberculosis
The re-emergence of multidrug-resistant tuberculosis (MDR-TB) has brought to light the importance of screening effective novel drugs. In the present study, in vitro activities of different snake (Naja naja, Bungarus fasc...
Phytochemical, organoleptic and ferric reducing properties of essential oil and ethanolic extract from Pistacia lentiscus (L.)
Objective: To study the phytochemical composition and organoleptic properties of Pistacia lentiscus (L.) (P. lentiscus) from Algeria and to investigate the antioxidant activities of its essential oil and ethanolic extrac...