Detection of Multidrug Resistant Gram Negative Bacilli in Type II Diabetic Foot Infections
Journal Title: International Journal of Medical and Health Sciences - Year 2013, Vol 2, Issue 2
Abstract
Diabetes is a major health challenge not only in the developed countries but also in the developing nation like India .Diabetic patients usually suffer from foot ulceration leading to polymicrobial infections. Frequent infections with drug resistant organisms will not respond to penicillin & cephalosporins .Therefore study was conducted in the Dept. of Microbiology, Dr. D.Y.Patil Hospital &Research centre, Kadamwadi ,Kolhapur to find out multidrug resistance in gram negative bacilli mediated through Extended Spectrum β- lactamase (ESBL) & AmpC β-lactamase in type II diabetic foot infections during period Jan 2010 to June 2011.Total 100 clinical samples from wounds in 100 diabetic foot patients were studied. Total no. of isolates found were 108.Out of these 63 were gram negative bacilli (GNB) (58.3%) & 45 were gram positive cocci (GPC)(41.66%).GNB isolates were processed further for study. Antibiotic sensitivity testing was done by Kirby Bauer disk diffusion method .Results were recorded as per Clinical Laboratory Standard Institute – CLSI guidelines. ESBL production was confirmed by Double Disk Synergy Test (DDST) & Minimum Inhibitory Concentration (MIC).Organisms showing resistance to β-lactamase inhibitors like clavulanic acid were screened for AmpC β-lactamase production by Disk Antagonism Test (DAT) & confirmed by AmpC Disk Test. Out of 63 GNB isolates 21 were ESBL producers (33.3%) & AmpC β-lactamase was found in 13 isolates (20.63%).All ESBL producing strains were sensitive to β-lactamase inhibitors like clavulanic acid. All AmpC producers were resistant to β-lactamase inhibitors but were sensitive to Imipenem.
Authors and Affiliations
Anjali S. Jog,P. G. Shadija, S. J. Ghosh
Keywords
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