Detection of platelet bacteria based on cultomics and metagenomics
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 11
Abstract
Objective To explore the composition of culturable bacteria in platelets through bacterial culturomics and verify the results of culturomics and metagenomics to improve the detection rate of bacteria in platelets. Methods Platelet samples from 6 healthy people were collected. Eight kinds of culture media were placed in aerobic conditions and 12 kinds of culture media were placed in anaerobic conditions for large-scale culture and isolation of bacteria in platelets. The isolated single colony was identified by 16S rRNA gene sequencing. The bacterial abundance of healthy human platelet microbiome was analyzed by metagenomic sequencing, and the cultivable bacterial species in platelets was confirmed based on metagenomic and culturomics results. Results A total of 90 strains of bacteria belonging to 3 phylums, 5 classes, 5 orders, 7 families, 9 genus and 23 species were isolated from 6 platelet samples by culturomics. Among them, the strains with more monoclonal clones at the species level were Brevundimonas aurantiaca (16.7%), Bacillus sp. Y1 (15.6%), Cutibacterium acnes (14.4%) and Brevibacillus brevis (13.3%). The platelet samples sequenced by mNGS showed that the abundance values of Proteobacteria, Firmicutes and Actinobacteria were high. The bacteria detected by both culturomics and metagenomic sequencing methods were as follows: Firmicutes: Bacillus sp. Y1, B. thuringiensis, B. cereus, B. mobilis, B. velezensis, Staphylococcus epidermidis, and Brevibacillus brevis; Actinobacteria: Cutibacterium acnes; Proteobacteria: Escherichia coli and Delftia tsuruhatensis. Conclusion The mutual validation of culturomics and metagenomics has identified some bacteria, proving that bacteria exist in platelets.
Authors and Affiliations
Mengyi ZHAO, Anqing LIU, Yuwei ZHAO, Xia RONG, Zhengang SHAN, Zhan GAO, Yang HUANG, Miao HE
Keywords
Related Articles
- EP ID EP738682
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.11.002
- Views 53
- Downloads 0
The Progress of Red Blood Cells Based Drug Delivery Systems
Red blood cells(RBCs), as the most abundant types of blood cells in the blood, have several advantages as a drug delivery system due to their ultra-long circulation half-life, good biocompatibility, and biodegradability....
Effect of storage time on the chemokine storage and chemokine scavenging function of erythrocyte atypical chemokine receptor 1
Objective To determine the effect of storage time on the chemokine storage and chemokine scavenging function of erythrocyte atypical chemokine receptor 1(ACKR1). Methods Samples from six bags of red blood cells product,...
Antigen distribution frequency of Han and Tujia polyhemia systems in Chongqing
[Objective] To analyse the distribution of antigen phenotypes in the Rh, MNS and Kidd blood group systems of Han and Tujia blood donors in Chongqing, and to provide data support for the establishment of an expanded blood...
Blood group changes in acute myeloid leukemia and their relationship with therapeutic effect
Objective To explore the blood group changes of two acute myeloid leukemia patients with suspected O type, and their relationship with the therapeutic effect. Methods Serological analysis of ABO blood group of patients w...
Application progress of hemoglobin-based oxygen carriers (HBOCs) in tumor therapy
Most solid tumors suffer from inadequate blood perfusion and oxygenation, leading to a hypoxic microenvironment that accelerates tumor progression and adversely impacts prognosis. Thus, improving oxygenation in tumor tis...