Detection of platelet bacteria based on cultomics and metagenomics
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 11
Abstract
Objective To explore the composition of culturable bacteria in platelets through bacterial culturomics and verify the results of culturomics and metagenomics to improve the detection rate of bacteria in platelets. Methods Platelet samples from 6 healthy people were collected. Eight kinds of culture media were placed in aerobic conditions and 12 kinds of culture media were placed in anaerobic conditions for large-scale culture and isolation of bacteria in platelets. The isolated single colony was identified by 16S rRNA gene sequencing. The bacterial abundance of healthy human platelet microbiome was analyzed by metagenomic sequencing, and the cultivable bacterial species in platelets was confirmed based on metagenomic and culturomics results. Results A total of 90 strains of bacteria belonging to 3 phylums, 5 classes, 5 orders, 7 families, 9 genus and 23 species were isolated from 6 platelet samples by culturomics. Among them, the strains with more monoclonal clones at the species level were Brevundimonas aurantiaca (16.7%), Bacillus sp. Y1 (15.6%), Cutibacterium acnes (14.4%) and Brevibacillus brevis (13.3%). The platelet samples sequenced by mNGS showed that the abundance values of Proteobacteria, Firmicutes and Actinobacteria were high. The bacteria detected by both culturomics and metagenomic sequencing methods were as follows: Firmicutes: Bacillus sp. Y1, B. thuringiensis, B. cereus, B. mobilis, B. velezensis, Staphylococcus epidermidis, and Brevibacillus brevis; Actinobacteria: Cutibacterium acnes; Proteobacteria: Escherichia coli and Delftia tsuruhatensis. Conclusion The mutual validation of culturomics and metagenomics has identified some bacteria, proving that bacteria exist in platelets.
Authors and Affiliations
Mengyi ZHAO, Anqing LIU, Yuwei ZHAO, Xia RONG, Zhengang SHAN, Zhan GAO, Yang HUANG, Miao HE
Keywords
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- EP ID EP738682
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.11.002
- Views 41
- Downloads 0
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