DETECTION OF SENSITIVE FOOD PATHOGENS IN BANANA, COLD MEAT AND MILK BY PCR AMPLIFICATION BASED TECHNIQUE  

Journal Title: Journal of Biological and Scientific Opinion - Year 2014, Vol 2, Issue 5

Abstract

This study was carried out to detect Escherichia coli, Klebsilla spp. and Streptococcus spp. isolated from food samples (banana, cold meat and milk). The homogenized food samples were subjected to biochemical and molecular techniques. Specific primers for virulent genes of three different bacterial strains were designed and subjected to polymerase chain reaction. PCR reaction was performed with human primers also in order to confirm the nonspecific amplification. Varying concentrations of DNA (100 ng, 50 ng, 20 ng, 5 ng and 1 ng) were chosen from each bacterial strain and PCR was carried out. It was observed that E. coli and Streptococcus were active in all the concentrations including 1 ng. However, Klebsiella did not produce amplification at 1 ng. The results obtained suggest that E. coli and Streptococcus could be detected and remain active even in 1 ng of the DNA sample. 

Authors and Affiliations

Brindha Durairaj, Kirupa Devaki , Santhoshkumar Muthu

Keywords

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DETECTION OF SENSITIVE FOOD PATHOGENS IN BANANA, COLD MEAT AND MILK BY PCR AMPLIFICATION BASED TECHNIQUE  

This study was carried out to detect Escherichia coli, Klebsilla spp. and Streptococcus spp. isolated from food samples (banana, cold meat and milk). The homogenized food samples were subjected to biochemical and molecul...

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  • EP ID EP99954
  • DOI 10.7897/2321-6328.02566
  • Views 120
  • Downloads 0

How To Cite

Brindha Durairaj, Kirupa Devaki, Santhoshkumar Muthu (2014). DETECTION OF SENSITIVE FOOD PATHOGENS IN BANANA, COLD MEAT AND MILK BY PCR AMPLIFICATION BASED TECHNIQUE  . Journal of Biological and Scientific Opinion, 2(5), 292-297. https://europub.co.uk/articles/-A-99954