Detection of Seroprevalence and Baseline Titre of Brucella Agglutinins in Blood Donors of Dhule District
Journal Title: Indian Journal of Pathology: Research and Practice - Year 2017, Vol 6, Issue 3
Abstract
Introduction: Brucellosis is a major zoonosis in the developing countries including India. The reported incidence of human brucellosis in endemic areas varies widely, from <0.01 to >200 per lakh population. Despite being endemic in many developing countries, brucellosis remains underdiagnosed and underreported. Interpretation of the serological tests is often difficult in endemic areas due to presence of antibodies against Brucella in high proportion of the population . Hence estimation of normal baseline titers of Brucella agglutinins in healthy individuals residing in endemic areas and the cutoff values are necessary for the serodiagnosis of brucellosis. AIM: To determine the baseline titre of brucella agglutinins, amongst apparently healthy blood donors in Dhule, Maharashtra, India. Materials and Methods: A total of 480 serum samples were analyzed by RBPT and STAT method for detection of Brucella Agglutinins over period of 6 months from July 2016 to December 2016. These agglutination tests were performed according to manufacturer‘s guidelines by using commercial kits containing stained Brucella abortus Antigen suspension. Result: Of these 480 samples 32 (6.7%) were positive for Brucella agglutinins by RBPT. Of these 32 RBPT positive samples 22(4.6%) showed agglutination by STAT. Amongst 22 STAT positive samples 15 samples were found positive for Brucella agglutinins at 1:40 and 7 samples were positive at 1:80. Conclusion: As RBPT is very sensitive test and its results should be confirmed by STAT. Since the base line titre of 1:80 by STAT was demonstrated in healthy individuals, titres 1:160 and above should be considered significant along with consistent clinical features.
Authors and Affiliations
Ahire Karuna Ravindra
Keywords
Related Articles
- EP ID EP466299
- DOI 10.21088/ijprp.2278.148X.6317.7
- Views 156
- Downloads 0
Study of Spectrum of Infections on Cervicovaginal Smears in a Rural Setting
Background: Cervical infections are commonly encountered problems occurring in the women of the reproductive age group. Pap smear, a screening test for carcinoma cervix is a simple, quick test that can also be used for d...
Histopathologic Spectrum of Prostatic Diseases and Prostate Specific Antigen Level
Aim: To delineate the histopathological pattern of prostate diseases and to correlate prostate speci c antigen values and histomorphological features. Methods and materials: A retrospective review was made of all prosta...
A Study of Platelet Indices In Healthy And Sick Neonates
Introduction: The platelet number alone does not give a complete picture of platelet maturity and function. Therefore, platelet indices have been the subject of intensive study in recent years, but they have not been fir...
An Analytical Study on the Various Histopathological Features of 250 Cases of Meningiomas and its Relation to the Behaviour of the Tumour
Context: Meningiomas are one of the most common primary central nervous system neoplasms. They occur most commonly in female patients being most common in the fourth and fifth decades of age. They may be of intraspinal o...
Histopathological Study of Mast Cells in Leprosy
Context: Mast cells have a multifunctional capacity. Their role in immunity is well known. Mast cells act as local immune modulators with a capacity to co-ordinate between pro and anti-inflammatory response. Leprosy is c...