DETERMINATION, ISOLATION, AND IDENTIFICATION OF AUCUBIN AND VERBASCOSIDE IN THE LEAVES OF IRAQI PLANTAGO LANCOLETA L. USING DIFFERENT DETECTING METHODS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2019, Vol 11, Issue 2
Abstract
Objective: Plantago lanceoleta L. (ribwort plantain) is one of the important medicinal herbs which is widespread fortune available in Iraq, that have a wide range of medicinal properties. The aim of this work was to determine, isolate and identify verbascoside and aucubin in Iraqi P. lanceoleta L. by using different chromatographic and spectrometric methods. Methods: Verbascoside and aucubin were isolated and quantified by preparative TLC, and then they were determined by the high-performance thin-layer chromatography (HPTLC) fingerprinting. Aucubin and catalpol in the plant extract were analyzed by liquid chromatography-mass spectrometry (LC-MS); aucubin and verbascoside that isolated from the plant sample were examined by fourier-transform infrared spectroscopy (FT-IR) and LC-MS, respectively. Results: The result showed that the Iraqi P. lanceoleta L. contains 1.74 percent (verbascoside) and 0.24 percent (aucubin) of dry powdered leaves. Each TLC-isolated compound showed a single spot on the HPTLC plate, which give an idea about the purity of the isolated compound. Aucubin (with catalpol) and verbascoside both are detected by LC-MS in different ionization mode. Many functional groups were identified in the TLC-isolated aucubin by FT-IR. Conclusion: The Iraqi P. lanceoleta L. showed a high content of verbasoside, and it is a very rich source for this compound, which can be easily isolated by TLC and subjected to many pharmacological studies. The extract of the young leaves of this plant gave a little amount of aucubin, and it is easy to obtain a higher content from the older leaves.
Authors and Affiliations
Hasan A. Khalaf, Ibrahim S. Abbas, Amani A. Tawfeeq, Monther F. Mahdi
Keywords
Related Articles
- EP ID EP566040
- DOI 10.22159/ijpps.2019v11i2.30566
- Views 71
- Downloads 0
IN VITRO AND IN SILICO ANTIMICROBIAL STUDY OF STANNANE OF PYRIDOXAL 5-PHOSPHATE
Objective: The main objective of this research work is to synthesize a novel stannane of pyridoxal 5-phosphateand to study its antibacterial property.Methods: Conventional method was used to synthesize the stannane of py...
IN VITRO ANTIOXIDANT ACTIVITY OF <em>PROSOPIS CINERARIA</em> LEAVES AGAINST H<sub>2</sub>O<sub>2</sub> INDUCED OXIDATIVE STRESS IN GOAT LIVER SLICES
Objective: To evaluate the antioxidant status of goat liver slices treated with standard oxidant H2O2in the presence and the absence of methanolic leaf extract of Prosopis cineraria.Methods: Precision-cut goat liver slic...
PHISHERS ATTACK ON THE RESEARCHERS FOR FINANCIAL GOALS IN PHARMACEUTICAL AND MEDICAL OPEN ACCESS JOURNALS
This is a letter.
ANTIFERTILITY ACTIVITY OF β-SITOSTEROL ISOLATED FROM BARLERIA PRIONITIS (L.) ROOTS IN MALE ALBINO RATS.
Objective: Plant–derived male antifertility agents need to address the basic requirements of fertility suppression without affecting libido. This study was undertaken to evaluate the male antifertility potential of the...
SYNTHESIS AND PHARMACOLOGICAL EVALUATION OF CERTAIN SCHIFF BASES OF OCTAHYDRO-1H-PYRROLO [3, 4-B] PYRIDINE DERIVATIVES
Objective: Synthesis, characterization and biological screening of some new 1,6-disubstituted Octahydro-1H-pyrrolo[3,4-b]pyridine Schiff base (13a-n) derivatives.Methods: The scaffold of Octahydro-1H-pyrrolo [3,4-b]pyrid...