ANTIFERTILITY ACTIVITY OF β-SITOSTEROL ISOLATED FROM BARLERIA PRIONITIS (L.) ROOTS IN MALE ALBINO RATS.
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 5
Abstract
Objective: Plant–derived male antifertility agents need to address the basic requirements of fertility suppression without affecting libido. This study was undertaken to evaluate the male antifertility potential of the β-sitosterol (BS) which is an active component isolated from the methanolic root extract of Barleria prionitis in the male albino rats.Methods: β-sitosterol was extracted using silica gel column chromatography from the methanolic root extract of Barleria prionitis and characterized by IR and NMR spectral analysis. Extracted BS was further used to determine its antifertility activity. The rats were orally administered olive oil (Group-I, control), BS at the dose level of 5 (Group II), 15 (Group III) and 25 mg/kg body weight (Group IV) for 60 d. Body weight was measured weekly. At the end of the experimental duration, treated males were sacrificed and subjected to biochemical, hormonal and sperm analysis. Fertility was assessed by mating treated rats with normally cycling virgin females.Results: Average weight of reproductive organs, serum levels of Testosterone, Follicular stimulating hormone (FSH) and Luteinizing hormone (LH), levels of Protein, Ascorbic acid, Glycogen, Fructose, sperm motility and sperm density was decreased significantly as compared to control group. While, Cholesterol level was increased significantly. Fertility percentage was also decreased in treated groups. Body weight remained unchanged.Conclusion: The results show that β-sitosterol from the roots of Barleria prionitis causes suppression of spermatogenesis and fertility thereby suggesting the potential of β-sitosterol for the development of male contraceptive which has very limited options available.Keywords: Libido, Fertility, Active component, NMR, Sperm, Testosterone, Contraceptive, SpermatogenesisÂ
Authors and Affiliations
Kamini Singh, R. S. Gupta
Keywords
Related Articles
- EP ID EP577616
- DOI -
- Views 58
- Downloads 0
DISEASE PREVENTION FUNCTIONAL FOODS FROM THE HIGH POTENTIAL MMP-2 INHIBITION ACTIVITY OF PINEAPPLE JUICE GRANULE
Objective: This study aimed to compare total phenolic acid and protein contents as well as free radical scavenging, enzyme activities of fresh and fermented pineapple juice. Methods: The dried pineapple juice, prepared...
ESTIMATION OF CELECOXIB IN HUMAN PLASMA BY RAPID AND SELECTIVE LC-MS/MS METHOD FOR A BIOEQUIVALENCE STUDY
Objective: A selective, sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay has been developed for the determination of celecoxib (CXB) in negative ionization mode.Methods: Celecoxib and c...
A RAPID STABILITY INDICATING HPLC-METHOD FOR DETERMINATION OF NORETHISTERONE ACETATE IN PLASMA, TABLETS AND IN A MIXTURE WITH OTHER STEROIDS
Objectives: This study reports about a simple, rapid and accurate, routine-HPLC method for quantification of Norethisterone acetate (NA) in bulk powder, tablets and plasma.Methods: The chromatographic separation is carri...
SYNTHESIS, ANTITUMOR ACTIVITY, PHARMACOPHORE MODELING AND QSAR STUDIES OF NOVEL PYRAZOLES AND PYRAZOLO [1, 5-A] PYRIMIDINES AGAINST BREAST ADENOCARCINOMA MCF-7 CELL LINE
Objective: The present work aimed to synthesize New series of pyrazoles 3 and pyrazolo[1,5-a]pyrimidines 5, 7, 9 in order to evaluate their antiproliferative activity against human breast adenocarcinoma MCF-7cell line an...
COMPARATIVE ANALYSIS OF THE IN VITRO ANTIOXIDANT ACTIVITY AND POLYPHENOLIC CONTENT OF SUCCESSIVE EXTRACTS OF NYCTANTHES ARBOR-TRISTIS LINN.
Objective: Present work comprehensively evaluates the antioxidant capacity, total phenolic content (TPC) and total flavonoid content (TFC) of four successive extracts of various parts of Nyctanthes arbor-tristis Linn. at...