Determination of antibacterial activity of Green Coffee Arabica bean extract on Multidrug resistance Pseudomonas aeruginosa (ATCC 27853)
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2018, Vol 8, Issue 12
Abstract
The emergence of drug resistant pathogens becomes a crucial problem for infectious diseases worldwide. Among these bacteria, P.aeruginosa is one of which highly resists to many currently used drugs and becomes a major concern in public health. Our study aims to assess an in vitro antibacterial activity of green coffee Arabica bean aqueous extract against the standard P.aeruginosa (ATCC 27853). The standard bacteria, P.aeruginosa (ATCC 27853) were prepared by sub-culturing a loopful of each strain in nutrient agar at 37°C for 24 hr. The turbidity of bacterial suspension was adjusted to 0.5 McFarland’s standard giving a bacterial load of about 1 × 108 CFU/ ml . Antimicrobial susceptibility was done by Kirby Bauer technique and Zone of inhibition was measured to the nearest millimeter using scale. Aqueous extracts of coffee were obtained by a coffee brewing procedure and the antibacterial activity of green coffee Arabica bean extract was determined by disc diffusion method. Green coffee Arabic bean aqueous extract showed the strongest antimicrobial activity against Multidrug-resistant P.aeruginosa (ATCC 27853) with minimum inhibitory concentrations of 0.2 mg/ml and MBC of 0.25 mg/ml.
Authors and Affiliations
Noha. MEL Sayed, Mada. SALharthi, Noor. MAllehyani, Asrar. NAlotaibi
Keywords
Related Articles
- EP ID EP421861
- DOI -
- Views 68
- Downloads 0
Antibiotic resistance patterns of methicillin-resistant Staphylococcus aureus (MRSA) isolated from chronic skin ulcer of patients in Kaduna state, Nigeria.
The aim of this study is to investigate the antibiotic resistance patterns of methicillin - resistant Staphylococcus aureus (MRSA) isolated from out - patients with chronic skin ulcer in four different ho...
Human cataract prevalence in Thiruvananthapuram district of Kerala state
Aim:-Hospital based study from April 2005 to March 2011 conducted among the population to determine the prevalence of cataract in Thiruvananthapuram district of Kerala state of India. Methodology:-The data’s colle...
Growth Performance, Carcass Characteristics, Lipid Profiles And Hematological Responses Of Broiler Finishers Fed With Leaf Meals Derived From ‘Ntong’ (Ocimumgratissimum) And ‘Utasi’ (Gongronemalatifolium)”
There is a need to identify new sources of poultry fodder in order to meet the growing demand for animal protein in the developing world. To this end, experiments were conducted to determine how the inclusion of...
Development and Validation of the HPLC Method for the Analysis of Ametridione in Bulk and Comercial Dosage Forms
A simple, economic, selective, precise, and accurate High Performance liquid Chromatographic method for the analysis of Ametridione in bulk and commercial formulations was developed and validated in the present s...
A Cross Sectional Study of Ethnic Differences in Occurrence and Severity of Adverse Drug Reactions (ADRs) Due to NSAIDs in Sikkim
Non-steroidal anti-inflammatory drugs are the most widely used “over the counter” medication all over the world despite their complications in different major organs. Present studies envisaged for knowing the occur...