Determination of antibacterial activity of Green Coffee Arabica bean extract on Multidrug resistance Pseudomonas aeruginosa (ATCC 27853)
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2018, Vol 8, Issue 12
Abstract
The emergence of drug resistant pathogens becomes a crucial problem for infectious diseases worldwide. Among these bacteria, P.aeruginosa is one of which highly resists to many currently used drugs and becomes a major concern in public health. Our study aims to assess an in vitro antibacterial activity of green coffee Arabica bean aqueous extract against the standard P.aeruginosa (ATCC 27853). The standard bacteria, P.aeruginosa (ATCC 27853) were prepared by sub-culturing a loopful of each strain in nutrient agar at 37°C for 24 hr. The turbidity of bacterial suspension was adjusted to 0.5 McFarland’s standard giving a bacterial load of about 1 × 108 CFU/ ml . Antimicrobial susceptibility was done by Kirby Bauer technique and Zone of inhibition was measured to the nearest millimeter using scale. Aqueous extracts of coffee were obtained by a coffee brewing procedure and the antibacterial activity of green coffee Arabica bean extract was determined by disc diffusion method. Green coffee Arabic bean aqueous extract showed the strongest antimicrobial activity against Multidrug-resistant P.aeruginosa (ATCC 27853) with minimum inhibitory concentrations of 0.2 mg/ml and MBC of 0.25 mg/ml.
Authors and Affiliations
Noha. MEL Sayed, Mada. SALharthi, Noor. MAllehyani, Asrar. NAlotaibi
Keywords
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