Determination of conditionally therapeutic dose of dry extract from Salat Crop leaves on a model of tetrachlormethane lesion of liver in rats
Journal Title: Український біофармацевтичний журнал - Year 2019, Vol 60, Issue 3
Abstract
Topicality. The search for natural remedies that would exhibit antiseptic, anti-inflammatory, detoxification, antioxidant, choleretic, hepatoprotective actions and positively influenced the entire body in order to create new medicines based on them is one of the pressing issues of modern pharmacology and pharmacy. Plants used for food can serve as a basis for new medicines. Aim. To establish a conditionally therapeutic dose of the extract from lettuce leaves in the experiment with simulated tetrachloromethane hepatitis in rats. Materials and methods. The experiments were carried out on white outbred male rats, who were simulated toxic liver damage with carbon tetrachloride (twice (every other day) at a dose of 1.0 ml/kg body weight). Euthanasia was performed using sodium thiopental on the fourth day after the introduction of carbon tetrachloride in compliance with all rules for working with vertebrate animals. For the study, extract doses of 50 mg, 100 mg, 150 mg and 200 mg per kilogram of body weight of the animal were used. The activity of lipid peroxidation was assessed by the content of TBAactive products, the activity of membrane-destructive processes - by the activity of aminotransferases (AsAT and AlAT), the state of the antioxidant system by the content of CP and catalase activity. Results and discussion. It was noted that the lesion of rats with carbon tetrachloride causes a significant (p ≤ 0.05) increase in serum levels of TBA-active products (2.1 times), ceruloplasmin (5.6 times) and aminotransferase activities. At the same time, a decrease in catalase activity is observed (by 5.8 times). The activity of AsAT and AlAT in the serum during this period increases almost equally – by 4 times. Significant changes in the studied parameters observed after application of a dose of 100 mg / kg of body weight of animals. Conclusions. A conditionally therapeutic dose of the extract is considered to be a dose of 100 mg/kg of body weight, which has a positive effect on the indices of lipid peroxidation, antioxidant system and cytolysis under conditions of modeled toxic hepatitis.
Authors and Affiliations
V. Pyda, L. S. Fira, P. Lykhatskyi
Keywords
Related Articles
- EP ID EP661102
- DOI 10.24959/ubphj.19.227
- Views 69
- Downloads 0
Study of biopharmaceutical solubility of quercetin and its solid dispersions
Topicality. Quercetin is a natural flavonoid, which has a variety of pharmacological properties, such as anti-inflammatory, antioxidant, anti-allergic, chemo-preventive, antiaggregate, etc. Despite the breadth of the pha...
Обоснование способа введения лизоцима гидрохлорида и аскорбиновой кислоты в состав резинок жевательных лечебных «Лизодент С»
Актуальность. В последние годы широкое распространение как системы доставки лекарств получили резинки жевательные лечебные. На кафедре заводской технологии лекарств НФаУ разрабатываются прессованные резинки жевательные п...
Вивчення впливу парацетамолу на функціональний стан нирок щурів за умов експериментальної інсулінорезистентності
Актуальність. Парацетамол (ПА) широко використовується в якості жарознижувального та аналгетичного засобу, однак особливості впливу ПА на органи і тканини в умовах інсулінорезистентності (ІР) вивчені недостатньо. Мета ро...
Зміни показників ендогенної інтоксикації у щурів за експериментального канцерогенезу та застосування ентеросорбції
Актуальність. Рак товстої кишки є однiєю з найбiльш поширених злоякiсних пухлин. На його частку припадає 45 % випадкiв захворювання на рак шлунково-кишкового тракту у жiнок i 60 % – у чоловiкiв. Виникнення пухлин супрово...
Гиалуроновая кислота: биосинтез и использование
Актуальность. Гиалуроновая кислота нашла широкое применение в медицине, хирургии, дерматологии, гинекологии, обладает антимикробными и противовирусными свойствами, а за счет высокой молекулярной массы является пролонгато...