Determination of fibrinogen content in cryoprecipitated antihemophilic factor by fluorescence quenching method
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 12
Abstract
Objective To establish a new method for the determination of fibrinogen content in cryoprecipitated antihemophilic factor. Methods Fibrinogen (Fib) could bind with sheep anti-human fibrinogen (anti-Fib) specifically and further form antigen-antibody complex. When the Fib was present in the solution, the fluorescence of fluorescein isothiocyanate (FITC) labeled on the anti-Fib (FITC-anti-Fib) was quenched due to the formation of immune complex. The fluorescence quenching degree of FITC-anti-Fib was positively correlated with Fib concentration (cFib) in a certain concentration range. Results The linear relationship between fluorescence quenching degree [(I0-I)/I0] of FITC-anti-Fib and ln(cFib) was (I0-I)/I0=15.53ln(cFib)+ 80.79 (R2=0.99) when the cFib was in the range of (0.007 8-0.560 0) g/L. The recovery of Fib was (96.77-102.43) %. When the method was applied to determine Fib at high, medium, and low concentrations, the obtained intra-day variation coefficients were 0.31%, 0.56%, and 0.49%, respectively, and the inter-day variation coefficients were 3.81%, 3.06%, and 4.13%, respectively. There was no significant difference between the results measured by fluorescence quenching method and coagulation method (t=-0.075, P>0.05). Conclusion In this work, a new fluorescence method for the determination of Fib in cryoprecipitated antihemophilic factor was successfully established based on the specific combination of fib and FITC-anti-Fib. The method is simple and rapid. The obtained results were accurate and reliable by using this method to determine Fib.
Authors and Affiliations
Jing LAN, Na FENG, Wenjuan ZHANG, Jin DING, Peng PENG, Na LIU, Xin CAO, Hua XU
Keywords
Related Articles
- EP ID EP738428
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.12.005
- Views 45
- Downloads 0
Correlation of D-dimer, serum potassium and thromboelasto-graphy parameters with progressive hemorrhagic injury
Objective To investigate the correlation between D-dimer, serum potassium and thromboelastography parameters and progressive hemorrhagic injury (PHI) after brain injury. Methods The data of 209 patients with traumatic br...
Effect of X-ray irradiation on the inactivation of lymphocytes and the quality of apheresis platelets
Objective To investigate the inactivation function of 25Gy X-ray irradiation on apheresis platelets’ lymphocytes and its effect on the quantity of apheresis platelets(AP). Methods Twenty healthy voluntary AP donors from...
Analysis of confidential unit exclusion status of voluntary blood donors in Hefei
Objective To explore the status of confidential unit exclusion(CUE) status among voluntary blood donors in Hefei, so as to provide scientific basis for blood safety. Methods From 2012 to 2021, 57 out of the 1 158 272 vol...
Efficacy of autologous ozoneized blood transfusion in improving motor function in convalescent stroke patients
Objective To investigate the efficacy of autologous ozoneized blood transfusion(AOBT) in improving consciousness and motor function in convalescent stroke patients. Methods A total of 44 patients who were diagnosed as co...
Characterization of platelet-derived extracellular vesicles induced by differential platelet activation pathways
Objective To investigate the effects of platelet activation pathways on the characterization of platelet-derived extracellular vesicles(PEVs). Methods Whole blood from healthy donors was prepared into platelet suspension...