Effect of X-ray irradiation on the inactivation of lymphocytes and the quality of apheresis platelets
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 8
Abstract
Objective To investigate the inactivation function of 25Gy X-ray irradiation on apheresis platelets’ lymphocytes and its effect on the quantity of apheresis platelets(AP). Methods Twenty healthy voluntary AP donors from January to May 2021 in our center were selected, and 2 bags of AP were donated by each of them. The APs were divided into two groups to undergo X-ray and γ-ray irradiation for 10 min. Lymphocytes were separated from AP samples, before and after irradiation, by lymphocyte separation solution to analyze and compare the effect of X-ray and γ-ray on lymphocyte proliferation. The CD41b, CD62p, blood routine and pH of APs before and 1-day/3-day after irradiation were detected. SPSS statistical software was used to analyze and compare the differences between groups by independent sample t-test. Results After 25Gy X-ray and γ-ray irradiation, the inhibition rates of lymphocytes were (98.034±1.778)% and (97.882±1.915)%, respectively.Compared with the γ irradiation group, the difference of Plt, PDW, MPV, P-LCR, PCT, pH, CD41b and CD62p between 1-day and 3-day group after 25Gy X-ray irradiation showed not statistically significance (P>0.05). Conclusion 25Gy X-ray irradiation can effectively inactivate lymphocytes in APs, and the radiation effect was equivalent with γ-ray; at the same time, there was no significant influence on the quantity of APs.
Authors and Affiliations
Chen CHENG, Yijing CHEN, Yi ZHANG, Hailong ZHUO, Qun LUO
Keywords
Related Articles
- EP ID EP738317
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.08.010
- Views 59
- Downloads 0
Application of quality monitoring indicators of blood testing in blood banks of Shandong province
Objective To objectively evaluate the quality control level of blood testing process in blood banks through quantitative monitoring and trend analysis, and to promote the homogenization level and standardized management...
Characteristics analysis of HBV serological markers of NAT reactive blood donors under different HBsAg status
Objective To investigate the characteristics of HBV serological markers of NAT reactive blood donors under different HBsAg status. Methods NAT reactive samples, with HBsAg-, HBsAg+ /retest - and HBsAg+ by single reagent...
Construction and function validation of inducible immortalized gene integration vectors
[Objective] To construct inducible immortalization gene vectors for transfection into primary cells, enabling the establishment of a conditionally immortalized cell line that support their sustained cultivation and proli...
Following up and re-entry of HBV reactive blood donors in Yantai area
Objective To analyze the preliminary screening and follow-up testing data of HBV in Yantai area, and discuss the rationality of following up and re-entry program of HBV reactive blood donors. Methods Donors who were sing...
Predictive value of preoperative liver function for perioperative massive blood transfusion in patients undergoing ascending aorta surgery
Objective To explore the predictive value of preoperative liver function for massive blood transfusion (MBT) in patients undergoing ascending aorta surgery. Methods Data from 238 patients undergoing ascending aorta surge...