Determination of Minimum Inhibitory Concentration (MIC) of a PolyHexamethylene Biguanide (PHMB) Solution: A Potential Root Canal Irrigant
Journal Title: Annual Research & Review in Biology - Year 2017, Vol 15, Issue 2
Abstract
Background: A polymeric biguanide ‘PolyHexaMethylene Biguanide’ (PHMB) was evaluated for its Minimal Inhibitory Concentration (MIC) against ATCC reference laboratory strain of Enterococcus faecalis (E. faecalis). Methods: The MIC was determined using criteria’s laid down in DIN 58940-7 and 58940-8 and the corresponding supplementary sheets. Serial dilutions of PHMB were tested against E. faecalis in 96-well microtitre plates and MIC was read as the minimal concentration that allowed no visible growth. Results: The MIC for PHMB against tested organism was found to be 2 mg/L. Conclusion: Lower MIC value (2 mg/L) of PHMB solution against E. faecalis is an indicator of its higher potency against the said microorganism and paves way to further research in its potential use as a root canal irrigant.
Authors and Affiliations
Chandki Rita, Vineeta Nikhil, Sai Kalyan Surapaneni
Keywords
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- EP ID EP313341
- DOI 10.9734/ARRB/2017/34141
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