Determination of Minimum Inhibitory Concentration (MIC) of a PolyHexamethylene Biguanide (PHMB) Solution: A Potential Root Canal Irrigant
Journal Title: Annual Research & Review in Biology - Year 2017, Vol 15, Issue 2
Abstract
Background: A polymeric biguanide ‘PolyHexaMethylene Biguanide’ (PHMB) was evaluated for its Minimal Inhibitory Concentration (MIC) against ATCC reference laboratory strain of Enterococcus faecalis (E. faecalis). Methods: The MIC was determined using criteria’s laid down in DIN 58940-7 and 58940-8 and the corresponding supplementary sheets. Serial dilutions of PHMB were tested against E. faecalis in 96-well microtitre plates and MIC was read as the minimal concentration that allowed no visible growth. Results: The MIC for PHMB against tested organism was found to be 2 mg/L. Conclusion: Lower MIC value (2 mg/L) of PHMB solution against E. faecalis is an indicator of its higher potency against the said microorganism and paves way to further research in its potential use as a root canal irrigant.
Authors and Affiliations
Chandki Rita, Vineeta Nikhil, Sai Kalyan Surapaneni
Keywords
Related Articles
- EP ID EP313341
- DOI 10.9734/ARRB/2017/34141
- Views 110
- Downloads 0
Disentanglement of Staphylococcus aureus in Chicken by Enzyme-Linked Fluorescent Assay
This study is aimed to detect Staphylococcus aureus (S. aureus) in the chicken using conventional methods (biochemical tests), latex agglutination, and Vitek Immuno Diagnostic Assay System (VIDAS) system. These methods a...
Efficacy of Cashew Nutshell Extract against Termite Attack on Triplochiton scleroxylon Wood
This study investigated the efficacy of cashew nutshell extract against termite attack on Triplochiton scleroxylon wood in other to add to the quest for readily available and cost-effective pest control materials, specif...
Biochemical and Haematological Assessment of Croton tiglium Seeds Mixed with Animal Diet in Male Albino Rats
Aim: The present study evaluates the toxic effects of Croton tiglium seeds mixed with animal diet on plasma and blood parameters in male albino rats. Methodology: Eighteen adult albino male rats, of age 6-8 weeks with a...
Estimation of Stability of Arterial Pressure and Pulse at Changes of Geomagnetic Data and Atmospheric Pressure
This work analyses annual blocks of daily observations of pulse with arterial blood pressure (ABP) and interrelation between them. Dynamics of morning and evening parameters is compared, behavior of atmospheric pressure...
Antimicrobial Drug Resistance in Escherichia coli Including an O157:H7 Isolate from Feces of Healthy Goats in Grenada
Aim: To determine: the occurrence of Escherichia coli including the O157:H7 serotype in feces of healthy goats in 10 randomly selected farms in Grenada and the antimicrobial drug resistance in E. coli isolated from fece...