Determination of platyphylline hydrotartrate by luminescence quenching of the complex yttrium (iii) with rutin
Journal Title: Фармацевтичний журнал - Year 2018, Vol 3, Issue 3
Abstract
Drugs used in the form of salts of organic bases are often determined by the anionic portion of these salts. Thus, titrimetric, spectrophotometric, electrochemical, chromatographic and luminescent methods of analysis are proposed for the determination of various tartrate-ion preparations. The purpose of this study was to develop a method of luminescent determination of platyphylline hydrotartrate in dosage forms using a luminescent probe-yttrium complex (III) with rutin (Rut). The luminescent determination of platyphylline hydrotartrate is offered to use a complex of yttrium (III)–rutin in the presence of bovine serum albumin (BSA). It has been experimentally established that tartrate ions quench of luminescence intensity of the Y (III)–Rut complex in the presence of BSA. The spectral and luminescent properties of the Y (III)–Rut complex in the presence of BSA were studied. The luminescence spectrum of the Y (III)–Rut complex in the presence of BSA has a maximum at λ = 570 nm, in the presence of platyphylline hydrotartrate of luminescence intensity of the Y (III)–Rut complex decreases and the maximum luminescence shifts to the long wavelength region of the spectrum (λ = 590 nm). It was established that the maximum quenching effect of the luminescence intensity of the Y (III)–rutin complex in the presence of BSA could be observed at the pH 6.0–7.0, which was created in solution with the help of urotropine. It is known that the luminescence quenching may be caused by various processes, including reactions in the excited state, energy transfer, formation of complexes and collisional quenching. It can be assumed that the quenching effect of Y (III)–Rut complex due to the complexation reaction of Y (III) with platyphylline hydrotartrate, that leads to the destruction of the Y (III)–Rut complex. Luminescence quenching of the Y (III)–Rut complex in the presence of BSA by platyphylline hydrotartrate follows the Stern–Volmer relationship. The Stern–Volmer constant is 1 230 l/mol. The luminescence method of the determination of platyphylline hydrotartrate in the dosage form (solution for injection) was developed. The received results were verified by method of spiked samples. The method is based on quenching of a rutin's molecular luminescence in the complex of the Y (III)–rutin by tartrate ions associated with the protonated form of platyphylline.
Authors and Affiliations
S. V. Beltyukova, E. V. Malinka
Keywords
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- EP ID EP551684
- DOI 10.32352/0367-3057.3-4.18.05
- Views 76
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