Determination of Survival Rates of Bacteria in Contaminated Fresh Fruit Juice Samples
Journal Title: Journal of Pharmaceutical Research International - Year 2014, Vol 4, Issue 10
Abstract
Aims: To determine the survival rates of bacteria in contaminated fresh fruit juice samples Place and Duration of Study: Biosciences and Biotechnology Department, Babcock University, Ilisan Remo, Ogun State, Nigeria, between November, 2012 and May, 2013. Methodology: Freshly extracted juice samples were obtained from intact pineapple (Ananas comosus Merr.) and watermelon (Citrillus lanatus Thunb.) were pasteurized before being contaminated with Escherichia coli ATCC 25922 and Lactobacillus acidophilus. While the pH and the sugar contents were determined at interval after being contaminated with the bacterial strains, the contaminated juice samples were sampled for 150 min to determine colony forming unit per milliliter (cfu/ml) at different sampling time. Results: In pineapple juice, the log of concentration of E. coli was reduced from 6.452 at 0 min to 5.079 at 150min. In watermelon, the log of concentration of E. coli was reduced from 6.301 at 0min to 5.954 at 150min. While the log of concentration of L. acidophilus in pineapple juice was between 6.204 at 0 min and 6.262 at 150min, its log of concentration in watermelon juice ranged between 6.228 at 0 min and 6.291 at 150min. The pH was reduced to 2.9 and 3.7 by E. coli while L. acidophilus reduced the pH to 2.5 and 3.0 for pineapple and watermelon juices respectively. After 150min, the sugar contents of pineapple and watermelon juices decreased from 1.181 and 1.060mg/ml to 0.011 and 0.004mg/ml by the E. coli while L. acidophilus reduced the sugar contents to 0.003mg/ml for pineapple juice and 0.018mg/ml for watermelon juice. The reduction in the pH values of each of the fruit juices showed that the activities of each bacterial strain resulted in increase in the production of acid in the growth medium. Conclusion: The inability of coli form (E. coli) to survive in the fruit juices suggested that the fruit juices may not harbor and/or disseminate enteric pathogens if allowed to stay for a while before packaging.
Authors and Affiliations
Olufunmiso O. Olajuyigbe, Olujimi M. Akinwande, Otunola Adedayo
Keywords
Related Articles
- EP ID EP344935
- DOI 10.9734/BJPR/2014/7384
- Views 118
- Downloads 0
In vitro Proliferation and Wound Healing Effects of Narcissus tazetta L. Bulb on Primary Human Dermal Fibroblasts
Aims: Narcissus tazetta L. bulbs have been used in Traditional Persian medicine (TPM), as "Zaroor" for wound healing. Toward quality assurance of the plant, pharmacognostic and phytochemical studies, physicochemical char...
Antimicrobial Resistance Profile of Urinary Isolates from Patients with Indwelling Urinary Catheter Diagnosed of Benign Prostate Hyperplasia in Bida, Niger State, Nigeria
Antimicrobial resistance is increasing daily and a cause of major challenges in many countries. Indiscriminate antibiotic prescription is associated to a higher prevalence of antibiotic resistant bacteria. The incidence...
Self-medication with Antibiotics: Empirical Evidence from a Nigerian Rural Population
Background: Self-medication is a strong determinant of antimicrobial overuse as well as a causative of drug resistance. Irrational antibiotic use among patients has led to antibiotic resistance and serious health problem...
Antimicrobial Screening and Phytochemical Analysis of Elaeis guineensis (Ewe Igi Ope) against Salmonella Strains
Aim: The study aimed at determining the in vitro anti-salmonella effect of Elaeis guineensis on Salmonella species isolated from clinically suspected typhoid fever patients in FUTA, Nigeria. Study Design: The study eval...
Strategies for the Microbiological Testing of Cosmetic Gels
Background: Official methods for the microbiological testing of cosmetics are available; evidence is required for the suitability of procedure employed particularly while dealing with thick or viscous preparations such a...