Determination of tetracycline resistance genes in Vibrio cholerae O1 biotype El Tor serotype Inaba strains isolated from outbreaks occurred in Iran in 2013
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 5
Abstract
V. cholerae is the causative agent of potentially life threatening diarrheal disease named as cholera. Cholera is an endemic disease in Iran. Encountered increasing resistance of V. cholerae to commonly used antibiotics such as tetracycline has led to major challenges in the treatment of this disease .The present study was carried out to determine the prevalence of drug resistance as well as molecular bases of resistant V.cholerae strains which were isolated from patients in cholera outbreaks during summer of 2013 in Iran. Susceptibly testing was performed on V.cholerae strains isolated from stool of patients suffering from cholera in Iranian reference health laboratory by E -test MIC method as recommended by CLSI guideline. Antibiotic strips used included Ampicillin, Ciprofloxacin, Nalidixic acid, Cefixime , Tetracycline, Erythromycin and Trimethoprim-sulfamethoxazole .Regarding observed dominant pattern of tetracycline resistance comparing to results of previous years ,we decided to confirm the resistance by detecting the tetA , tetB and tetC by Polymerase chain reaction method. The results of antibiotic susceptibility testing revealed 100% resistance of isolated strains to tetracycline. Data obtained from PCR reaction on resistant strains for tetA, tetB and tetC showed that 45(44.1%), 37(36.2%) and 70(68.6%) were containing tetA, tetB and tetC gene respectively. Moreover, the frequency of tetA+tetB, tetA+C, tetB+tetC , tetA+tetB+tetC also were determined as 9(8.8%), 32(31.3%), 19(18.6%) and 8(7.8%) respectively. This study revealed the pattern of drug resistance distribution of isolates harboring tetA, tetB, tetC genes in relation to sex, age and nationality of patients and the cities where the cases were reported. A significant correlation was obtained between reported geographical incidence and drug resistant strains.
Authors and Affiliations
Azin Khany| Department of Microbiology, faculty of biosciences, Islamic Azad University, Tehran North Branch, Iran, Mitra salehi| Department of Microbiology, faculty of biosciences, Islamic Azad University, Tehran North Branch, Iran, Marjan Rahnamaye Farzami| Reference Health Laboratory, Ministry of Health and Medical Education, Tehran, IR Iran, Corresponding E.mail:marjan.farzami@gmail.com
Keywords
Related Articles
- EP ID EP11665
- DOI -
- Views 259
- Downloads 10
HOW DO MEDICAL STUDENTS LEARN? A STUDY FROM TWO MEDICAL COLLEGES IN SOUTH INDIA – A CROSS SECTIONAL STUDY
Introduction: "Learning style" is defined as an individual's preferred method for approaching learning and gaining knowledge. As a teacher, it is important to understand the different learning styles of the students in...
Simultaneous Detection of Methicillin- and Mupirocin-Resistant genes in Staphylococcus aureus Isolated with Multiplex PCR from Patients in the City of Darab, Fars Province, Iran
Background and Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important pathogens in hospitals. Hospital staffs can be one of the main sources of this organism in nosocomial infections...
A RARE CASE OF OSTEOGENESIS IMPERFECTA TYPE III
Osteogenesis imperfecta (OI) the most common genetic cause of osteoporosis is a generalized disorder of connective tissue, characterized by increased bone fragility, low bone mass, recurrent fractures & numerous extraos...
Factors Affecting the Decision of the Emergency Nurses on Critically Ill Patients
Nowadays, the hospital emergency department admits critically ill patients from pre-hospital emergency or other medical center, and it undertakes the duty of recording the patient's vital signs to enter the clinical dep...
Detection of Cyclospora cayetanensis Infections among Diarrheal Children Attending Pediatric Teaching Hospitalin Sulaimani City
The present study aimed to investigate the Cyclospora infection among children attended Pediatric Teaching Hospital in Sulaimani City and to determine its prevalence among other intestinal parasites. The study started f...