Determination of the content of the main components of the culture medium during the differentiation phase of cell suspensions of Coffea arabica cv. Catimor 9722
Journal Title: Biotecnología Vegetal - Year 2009, Vol 9, Issue 3
Abstract
Exhaustion of culture medium components and possible influence on the in vitro development of plants has been little studied. The current work was developed in order to know and determine contents of the main components in culture medium in the differentiation stage of cell suspensions of Coffea arabica cv. Catimor 9722, during 56 days of culture. Samples of 30 ml were taken every seven days, even from fresh culture medium than the renovated one. Then, content of sugars, 6-BAP and main ions were determined. During the first 21 days of culture and due to the hydrolysis of the sucrose, the two monosaccharide constituents (glucose and fructose) were present. These were completely exhausted at 56 days of culture. Content of 6-BAP in the culture medium diminished quickly during the first 21 days. Although a concentration of 2.75 mg.l -1 was added every seven days, it was not detected in the samples analyzed after 28 days of culture. Content of different ions was determined. Elements such as magnesium and chloride decreased slightly in the culture medium. Contrary happened to phosphate, potassium, calcium and sulphate. Content of ions such as ammonium and nitrate were analyzed and their relationship with the variations of pH too. During the first 21 days of culture there was a further depletion of nitrogen in ammonia form and a rapid decrease in pH. However, after 21 days of culture the first somatic embryos in globular stage were observed. This moment coincided with the beginning of a gradual increment of pH values and also the beginning of nitrogen depletion in nitrate form.
Authors and Affiliations
Manuel de Feria, Elio Jiménez, Raúl Barbón, Alina Capote, Maité Chávez, Elisa Quiala
Keywords
Related Articles
- EP ID EP683860
- DOI -
- Views 139
- Downloads 0
Effect of induced hydric stress with PEG 6000 on Stevia rebaudiana cv. ‘KH-IAN/VC-142’ in vitro growth
Drought is one of the most important abiotic stresses, since it limits the production and growth of Stevia rebaudiana (Bertoni) Bertoni. The objective of the present work was to determine the effect of water stress ind...
Determination by AFLP of the genetic stability of cassava plants obtained by somatic embryogenesis and organogenesis
AFLP techniques have been reported to determine the genetic stability at a molecular level of cassava plants propagated by several methods: somatic embryogenesis, organogenesis and field conditions. CMC-76, CEMSA 74-7...
In vitro establishment and multiplication of Curcuma longa L.
Turmeric is a very valuable plant as condiment, dye and due to its medicinal properties. Although results achieved in the in vitro propagation, rates of in vitro establishment and multiplication are low. The aim of this...
Effect of hydric stress induced by PEG 6000 on water content of Musa spp. ‘Grande naine’ (AAA) and ‘Pelipita’ (ABB) in vitro plants
Bananas and bananas are grown in the tropical and subtropical regions of the world, by hence they are adapted to good humidity conditions. The productivity of these crops is affected by abiotic factors such as drought,...
In vitro phosphate solubilization by Aspergillus and Penicillium strains and coffee plants growth promotion (2019)
The availability of phosphorus in the soil for the growth of plants can be increased by the action of phosphate solubilizing microorganisms. The objective of this work was to determine the potential of Aspergillus and...