Determination of the efficiency of different sorbents doses against fungi aflatoxin B1 and zearalenon in vitro
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2017, Vol 31, Issue
Abstract
Introduction. Microscopic fungi that produce mycotoxins is a constituent element of ecosystem. Mycotoxins contamination of feed can cause poisoning of animals as well as mycotoxicoses. Existing methods of contaminated feed processing are not enough effective and do not provide a solution of this issue. Recently, much attention is paid to the known methods of feed detoxication using different sorbents.The goal of the work. To study efficiency of different sorbents doses against mycotoxins zearalenon and aflatoxin В 1 in vitro.Materials and methods. In our research we used two samples of feed grains (wheat, corn), which had been investigated for the fungi contamination and the ability of fungi to produce mycotoxins with studying of sorption activity of different sorbents. In our research we used two sorbents: # 1 based on organic components zeolite, and # 2 based on Saccharomyces cerevisiae. The investigated doses were 100 mg/kg, 200 mg/kg, 300 mg/kg.Results of the study and discussion. It was conducted mycotoxicological research of grain and corn. After fungi isolation and identification two producers of mycotoxins were isolated - Aspergillus flavus - producer of aflatoxin В 1 and Fusarium graminearum - producer of zearalenon.As a result of the studies of sorbent #1, it was established 100% sorption activity against aflatoxin В1. The level of its sorption activity in the dose of 100 mg/kg was 60%, in the dose of 300 mg/kg - only 65%.Sorbent # 2 sorption activity against aflatoxin В1 was 85% in dose of 100 mg/kg. After dose increasing the sorption activity increased too, up to 90%. The level of its sorption activity against zearalenon in dose of 100 mg/kg was 70%, in dose of 200 mg/kg 85%, in dose of 300 mg/kg 90%.Conclusions and prospects for further research. It was detected that the most effective dose of sorbent # 1 with zeolite against aflatoxin B 1 was 100 mg per kg. This sorbent is not effective against zearalenon. The sample # 2 - sorbent based on Saccharomyces cerevisiae, showed the best sorption activity against both aflatoxin B 1 and zearalenon in dose 200 мg per kg.
Authors and Affiliations
Maryna Ruda
Keywords
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- EP ID EP266541
- DOI 10.31073/vet_biotech31-16
- Views 102
- Downloads 0
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