Investigation of antigenic affinity of streptococcus suis isolates in Ukraine
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2017, Vol 30, Issue
Abstract
Introduction. Streptococcus suis type 2 is an important pathogen for pig industry in many countries. Today are known more than 35 different capsular serotypes of S. suis. In the last years registered the significant growth of streptococcal infections prevalence. Considering of these, the problem of prevention and treatment of streptococcosis is serious and important for pig industry susteinability. The goal of the work was to investigate the antigenic affinity of Streptococcus suis isolates.Materials and methods. It was used 11 isolates of Streptococcus suis, negative and positive serums of blood. For investigations of antigenic properties it was used the inderect ELISA method with in-house prepared antigens. For isolation and characterization of strains and isolates, it was used standard bacteriological approaches and culture methods. Detection of the capsule was performed by Neifiield method. Results of research and discussion. As a result of the experiments, it was found that among the strains studied the most antigenically active were isolates 10, 11, 19 and 21, which had an average specificity coefficient for the reference strain from 1.60 to 1.75 and high antigenic activity for homologous and heterologous phenotypes of the pathogen (A specificity factor of 1.53 - 1.78). Avirulent isolate 16 exhibited low activity against virulent phenotypes with a specificity coefficient of 2.01 to 2.93. Conclusions and prospects for further research: Based on the data presented, it has been established that specific sera obtained against the corresponding S. suis antigens have specificity and can be used for typing the causative agent of streptococcosis in pigs. Among the strains studied, isolates 10, 11, 19 and 21 proved to be the most antigenically active, which should have an average specificity coefficient toward the reference strain from 1.60 toand high antigenic activity against homologous and heterologous phenotypes of the pathogen (specificity factor 1.53-1.78). Avirulent isolate 16 showed a low activity against virulent phenotypes with a specificity coefficient of 2.01 to 2.93.Therefore, further research antigenic properties of S. suis is an important direction of improving immunogenic potency of vaccines.
Authors and Affiliations
Alexander Tarasov, Maria Babkinа, Svitlana Tereschenko, Iryna Zotcenko, S. Krilenko
Keywords
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- EP ID EP272359
- DOI 10.31073/vet_biotech30-27
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