Determination of Vitamin B6, B9 and B12 in Halophytes by Solid Phase Extraction Followed by HPLC-UV
Journal Title: Journal of Pharmaceutical Research International - Year 2017, Vol 20, Issue 4
Abstract
In this work, solid phase extraction (SPE) was proposed for simultaneous determination of trace levels of vitamin B6, B9 and B12. High performance liquid chromatography with UV detection (HPLC-UV) was used. The experimental conditions including pH of sample and type and volume of extraction solvent were studied by one-factor-at-a time method. The limit of detection in the optimal conditions was 14.91, 10.03, and 7.25 ngkg-1 for vitamins B6, B9 and B12 respectively and relative standard deviations (RSD) of the analysis less than 8% (n= 3). Recoveries of vitamins B6, B9 and B12 were in the ranges of 80–93%. The proposed method was successfully applied for the simultaneous determination of vitamin B6, B9 and B12 in halophyte samples such as, Salicornia, Suaeda monoica, Suaeda aegyptiaca, Sueada vera and Sueada martima.
Authors and Affiliations
Narges Chamkouri, Mona Vafaeizadeh, Ayyub Mojaddami, Seyyed Abed Hosseini, Tahereh Momeni Isfahani, Aida Ghorbani Golkhajeh, Milad Janghorban Lariche, Seyed Mohammad Ali Malaekeh
Keywords
Related Articles
- EP ID EP313093
- DOI 10.9734/JPRI/2017/34730
- Views 122
- Downloads 0
Phytochemical and Biological Activities of the Wild Grape Fruit Extracts Using Different Solvents
Aims: To investigate the phytochemical, antioxidant and antibacterial activities of the solvent extracts of wild grape fruits in different colors. Study Design: The solvent extracts of wild grape (Ampelocissus martinii P...
Effect of Vitellaria paradoxa Stem Bark Ingestion on Kidney Functions in Wistar Rats
Background: Vitellaria paradoxa is used in the treatment of different diseases in several parts of Nigeria without regard for its safety. Aim: This study was aimed at investigating the effect of ingestion of Vitellaria...
X-ray Crystal Structure of COX-2 Enzyme as a Tool to Predict Active Sites of Bitter Taste Receptors
This commentary discusses a novel approach to be used in the design of active sites of bitter taste receptors, especially TAS2R14, by using the X-ray crystal structure of the enzyme COX-2 and chemical structures of commo...
Development of Anti-bacterial Ointment from Two Extracts of Curcuma longa L. and Aloe vera L.
Aim of this Study: This study aimed to extract, formulate and evaluate an antibacterial ointment from extracts of two Yemeni plants. Methods: The extraction of both Curcuma longa and Aloe vera was performed using alcohol...
Influence of Rhizophora apiculata Flavonoids on Chemical and Thermal Induced Nociceptive Models
Aims: Rhizophora apiculata is a traditional medicine used to treat pain, ulcer, and inflammation in southeast coast of India without scientific evidence. Therefore, we aimed to evaluate antinociceptive effect of R. apicu...