Determination of white blood cells in blood components. Experience of Tor Vergata transfusion service.
Journal Title: Biomedicine & Prevention - Year 2018, Vol 2018, Issue 1
Abstract
ABSTRACT. Background. The number of White Blood Cells (WBCs) that are contained in blood components is very important to guarantee a safe transfusion therapy (1, 3, 4). Objective. Our goal is to show that our Service is able to guarantee a high level of quality for blood components in conformity with the recommendation n R (95) 15 (2,7). Materials and methods. The number of WBCs has been evaluated in blood components by both analyzers and cytometer from September 2015 to November 2016. We have tested components from different Transfusion Services: Tor Vergata, Frosinone, Velletri and Campus Biomedico. Results. Analyzers have shown satisfactory results for Platelet (PLT) concentrates, but not for erythrocyte ones. Conversely, cytometer is not only as effective as analyzers in determining WBCs in platelet concentrates, but it is more effective than analyzers regarding Red Blood Cell (RBC) concentrates. Discussion. The two techniques have different sensibility and specificity: results indicate that our blood components are characterized by a high level of quality. Conclusion. Cytometer is a very expensive technique and very well-trained operators are needed: it is our opinion that is more appropriate to use analyzers for counting WBCs in platelet concentrates and cytometer in erythrocyte concentrates.
Authors and Affiliations
Colosimino Daniele, Danila Insalaco, Maddalena Antonelli, Gianpaolo Del Proposto, Alessandro Lanti, Gaspare Adorno
Keywords
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- EP ID EP336299
- DOI 10.19252/00000008E
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