Deterministic analysis of the gene regulation network involved in the expression and function of the transcription factor σ32 in Escherichia coli
Journal Title: Revista Cuarzo - Year 2017, Vol 23, Issue 2
Abstract
Motivation: The expression of σ 32 factor is a response of E. coli under thermal shock conditions. The mechanism of induction of chaperone proteins and thermal shock proteases has been well studied, but much of the regulation network of the σ32 factor is not fully understood. For this reason, inthis work a deterministic model of the network is proposed, which was solved by means of the tools present in Matlab® and then evaluated the influence of the transcription, translation and degradation speed on all concentrations of the species involved in the network, in order to determine which of these factors is the one that the cell possibly employs to regulate the expression of the factor σ32. Results: In the resolution of the ordinary equation system using the ode45 algorithm, it was corroborated that the mean life time of sigma 32 is 1 minute for the concentration in steady state. In addition, variations in rpoH transcription constants and o32 mRNA degradation in the network (k1, k2) were found to be non-significant in σ32 factor production, nor in the production of thermal shock proteins. On the other hand, from sensitivity analysis it was inferred that the k3 translation constant of σ32 RNA is the most critical speed for regulating the expression of the σ32 factor in thenetwork. Finally, with respect to k12 it is concluded that it is an important term in the stabilization of the σ32 factor. Availability and Implementation: The analysis was conducted at MatLab_R 2011a and the source code is found in the Supplemental Material
Authors and Affiliations
Clavijo Buriticá, D. , Mejía, B. , Rojas, L. , Sáenz, L. , & Yosa, J.
Keywords
Related Articles
- EP ID EP694613
- DOI https://doi.org/10.26752/cuarzo.v23.n2.300
- Views 141
- Downloads 0
Born alive with low weight at birth in Bogotá 2014-2016
Introduction. The World Health Organization (WHO) considers low birth weight a public health problem, since it increases the risk of noncommunicable diseases in later life and is the most frequent cause of neonatal morbi...
Profile of Autoantibodies in Adults With Primary Inmune Thrombocytopenia in an Institution of Bogota.
Introduction. A platelet count below 150,000 and normality in the remainder of the blood count are necessary to consider primary immune thrombocytopenia (ITP). Although the pathogenesis has not been...
Influence of Candida spp. respiratory tract colonization in chronic obstructive pulmonary disease in in-tensive care unit, Colombia, 2010-2015
Candida spp. is the most common agent isolated associated to hospital infection of fungal origin in the context of Intensive Care Unit (ICU),Chronic Obstructive Pulmonary disease (COPD), has a particular relevance as a f...
Alteration of mental state, a case report
The altered state of consciousness is an issue that can be related with several entities, diagnosis and therefore the appropriate handling relays on the adequate approach to the patient who comes to ER serv...
Cyanide Poisoning Secondary to Unintentional Dermal and Inhalation Exposure: Case Report and Literature Review
Introduction. Cyanide is a readily available chemical on the market used in various industries such as insecticide production, photography, and jewelry. The cases of poisoning due to this compound have a high mortality r...