Develop a solid-phase red cell adherence reagent kit for IgG unexpected alloantibody identification
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 10
Abstract
Objective To develop a solid-phase red cell adherence(SPRCA) reagent kit for unexpected alloantibody identification. Methods A solid-phase carrier for bounding red blood cell(RBC) was prepared by coating microstrip with succinimide, then panel cell suspensions were dispensed to microwells to produce RBC monolayers, after that, 1×PBS(pH7.40) was used to lysis the RBC monolayer to form stroma monolayer. Prior to the drying procedure, drying medium suspension was added to microwell to protect stroma monolayer. The solid-phase microplate was successfully produced after the stroma had been dried in refrigerator at 4℃. IgG anti-E and anti-Fya with low titer were tested every two months with the reagent kit to observe its stability. Seventy-two plasma samples containing unexpected antibodies and 152 normal plasma samples were determined with SPRCA reagent kit and column agglutination technique(CAT). Performances including sensitivity and specificity of the two reagents were compared by paired chi-square test. Results RBC stroma monolayer could be attached well on microwell bottom coated with succinimide. Drying medium suspension could provide effective protection for stroma monolayer. Both IgG anti-E and anti-Fya with low titer were determined well by the reagent kit during the storage period. Of the 72 positive samples, 70 were identified correctly by SPRCA kit and 71 by CAT [Sensitivity:97.22%(70/72) vs 98.61%(71/72)]. Of the 152 negative plasma samples, 149 were correctly detected by SPRCA kit and 152 by CAT [Specificity:98.02%(149/152) vs 100%(152/152)]. Paired chi-square test showed no significant differences between the two reagents (P>0.05). Conclusion A SPRCA reagent kit for IgG unexpected antibody identification had been developed successfully.
Authors and Affiliations
Hong LUO, Yuan SHAO, Jiali WANG, Zhen WANG, Guangping LUO
Keywords
Related Articles
- EP ID EP749841
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.10.012
- Views 22
- Downloads 0
Efficacy and safety of platelet-rich plasma for acute achilles tendon injury: a meta-analysis
Objective To evaluate the clinical efficacy and safety of platelet-rich plasma(PRP) in acute achilles tendon injury by meta-analysis. Methods Literature on clinical randomized controlled trial of PRP in the treatment of...
Clinical observation of autologous platelet-rich plasma eye drops in the treatment of dry eye syndrome
Objective To explore the effect of autologous platelet-rich plasma(PRP) in the treatment of dry eye syndrome. Methods A total of 64 patients with dry eye treated in the Ophthalmology Department of Jingdong Sino-American...
Application of quality control indicator system in blood banks of Shandong
Objective To establish an effective quality monitoring indicator system for blood quality control in blood banks, in order to analyze the quality control indicators for blood collection and supply, and evaluate blood qua...
Study on mitochondrial respiratory function in stored apheresis platelets using real-time energy metabolism detection technology
Objective To explore the optimal parameters for detecting mitochondrial respiratory function (MRF) in apheresis platelets (AP) using real-time energy metabolism detection technology, and to study the change of MRF in AP...
Study on transfusion-related acute lung injury caused by HLA-Ⅱ antibody
Objective To explore the risk factors of transfusion-related acute lung injury (TRALI). Methods The clinical symptoms, signs, imaging examinations, and laboratory test results of two patients with TRALI after blood trans...