Effect of RhD antigen expression intensity on preparation of low positive quality control products for transfusion compatibility test
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 6
Abstract
Objective To determine the reference red blood cells with weak agglutination intensity of low positive quality control products by comparing RhD antigen expression intensity difference according to the serological results. Methods The RhD(+ ) red blood cells were detected by microcolumn gel method with 1 500 times diluted anti-D typing reagent. The samples with weak and strong RhD antigen expression intensity were selected as the reference red blood cells for weak agglutination intensity of low positive quality control products, and verification was performed. Results Ten RhD(+ ) red blood cells were detected with diluted anti-D typing reagent, of which 8 were 1+ and 2 were ±. Red blood cells with agglutination intensity of 1+ were used as the benchmark to determine the maximum dilution ratio of anti-D typing reagent when their agglutination intensity was 1+. As the preparation standard of low positive quality control products, the agglutination intensity of red blood cells with low RhD antigen expression intensity was extremely weak ±, which was difficult to ensure the stability of its control limit properties. Based on red blood cells with agglutination intensity of ±, the maximum dilution ratio of anti-D typing reagent with agglutination intensity of 1+ was re-determined as the preparation standard of low positive quality control products, and the results met the requirements of quality control product setting. Conclusion Using red blood cells with low RhD antigen expression intensity as the benchmark to set the weak agglutination intensity of the low positive quality control products can avoid the loss of control due to the low target value.
Authors and Affiliations
Lu LI, Junjie WEI, Xiaolin SUN, Weixin WU, Ruiqi LIU, Haiyun LIU, Yinze ZHANG
Keywords
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- EP ID EP742789
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.06.014
- Views 35
- Downloads 0
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